LOCUS BBa_I13950 4632 bp DNA linear 25-Nov-2024 DEFINITION Exogenous LuxI Relaxation Oscillator Test Construct (I13940.I13941) ACCESSION BBa_I13950 VERSION BBa_I13950.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 1816..1847 /label=LVA CDS 1237..1815 /label=luxI misc_feature 1237..1854 /label=BBa_C0061 misc_feature 1855..1879 /label=Help:Barcodes CDS 1237..1854 /label=BBa_C0061 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 misc_feature 2662..2667 /label=stop misc_feature 2623..2661 /label=AAV misc_feature 1906..2667 /label=BBa_E0034 stem_loop 2687..2730 /label=stem_loop misc_feature 2676..2755 /label=BBa_B0010 terminator 2676..2755 /label=BBa_B0010 stem_loop 2771..2790 /label=T7 TE misc_feature 2797..2797 /label=stop polyA_site 2791..2804 /label=polya misc_feature 2764..2804 /label=BBa_B0012 terminator 2764..2804 /label=BBa_B0012 misc_feature 2813..2832 /label=LuxR/HSL promoter 2832..2837 /label=-35 promoter 2854..2859 /label=-10 misc_feature 2865..2865 /label=start misc_feature 2813..2867 /label=BBa_R0062 promoter 2813..2867 /label=BBa_R0062 misc_feature 2880..2883 /label=conserved RBS 2876..2887 /label=BBa_B0034 misc_feature 2894..2896 /label=start misc_feature 3677..3682 /label=2 misc_feature 3644..3676 /label=LVA CDS 2894..3643 /label=aiiA misc_feature 2894..3682 /label=BBa_C0060 misc_feature 3683..3707 /label=Help:Barcodes CDS 2894..3682 /label=BBa_C0060 misc_feature 3720..3723 /label=conserved RBS 3716..3727 /label=BBa_B0034 misc_feature 3734..4495 /label=BBa_E0024 stem_loop 4515..4558 /label=stem_loop misc_feature 4504..4583 /label=BBa_B0010 terminator 4504..4583 /label=BBa_B0010 stem_loop 4599..4618 /label=T7 TE misc_feature 4625..4625 /label=stop polyA_site 4619..4632 /label=polya misc_feature 4592..4632 /label=BBa_B0012 terminator 4592..4632 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga ctataatgat aaaaaaatcg 1261 gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg ttatcaagtg 1321 tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc agatgagtat 1381 gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt aagtggatgc 1441 tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc tgaattgctt 1501 ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt tgctgtaggt 1561 aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact atttgaagct 1621 atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac atcaacagca 1681 atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga caaagaaatt 1741 catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga acagtttaaa 1801 aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta ataactctga 1861 tagtgctagt gtagatctct actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 1981 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 2041 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc 2101 ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc 2161 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 2221 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 2281 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 2341 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 2401 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 2461 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag 2521 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 2581 accgccgccg ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac 2641 gaaaactacg ctgctgctgt ttaataatac tagagccagg catcaaataa aacgaaaggc 2701 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2761 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 2821 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagagaaaga 2881 ggagaaatac tagatgacag taaagaagct ttatttcgtc ccagcaggtc gttgtatgtt 2941 ggatcattcg tctgttaata gtacattaac accaggagaa ttattagact taccggtttg 3001 gtgttatctt ttggagactg aagaaggacc tattttagta gatacaggta tgccagaaag 3061 tgcagttaat aatgaaggtc tttttaacgg tacatttgtc gaagggcagg ttttaccgaa 3121 aatgactgaa gaagatagaa tcgtgaatat tttaaaacgg gttggttatg agccggaaga 3181 ccttctttat attattagtt ctcacttgca ttttgatcat gcaggaggaa atggcgcttt 3241 tataaataca ccaatcattg tacagcgtgc tgaatatgag gcggcgcagc atagcgaaga 3301 atatttgaaa gaatgtatat tgccgaattt aaactacaaa atcattgaag gtgattatga 3361 agtcgtacca ggagttcaat tattgcatac accaggccat actccagggc atcaatcgct 3421 attaattgag acagaaaaat ccggtcctgt attattaacg attgatgcat cgtatacgaa 3481 agagaatttt gaaaatgaag tgccatttgc gggatttgat tcagaattag ctttatcttc 3541 aattaaacgt ttaaaagaag tggtgatgaa agagaagccg attgttttct ttggacatga 3601 tatagagcag gaaaggggat gtaaagtgtt ccctgaatat atagctgcaa acgacgaaaa 3661 ctacgcttta gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagaaaga 3721 ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 3781 ggtcgagctg gacggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaggg 3841 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 3901 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc 3961 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 4021 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 4081 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 4141 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga 4201 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag 4261 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 4321 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 4381 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 4441 gctgtacaag aggcctgctg caaacgacga aaactacgct gctgctgttt aataatacta 4501 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 4561 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 4621 tctgcgttta ta //