LOCUS BBa_I13951 4632 bp DNA linear 22-Dec-2024 DEFINITION Exogenous AiiA Relaxation Oscillator Test Construct (I13942.I13943) ACCESSION BBa_I13951 VERSION BBa_I13951.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 1237..1239 /label=start misc_feature 2020..2025 /label=2 misc_feature 1987..2019 /label=LVA CDS 1237..1986 /label=aiiA misc_feature 1237..2025 /label=BBa_C0060 misc_feature 2026..2050 /label=Help:Barcodes CDS 1237..2025 /label=BBa_C0060 misc_feature 2063..2066 /label=conserved RBS 2059..2070 /label=BBa_B0034 misc_feature 2077..2838 /label=BBa_E0024 stem_loop 2858..2901 /label=stem_loop misc_feature 2847..2926 /label=BBa_B0010 terminator 2847..2926 /label=BBa_B0010 stem_loop 2942..2961 /label=T7 TE misc_feature 2968..2968 /label=stop polyA_site 2962..2975 /label=polya misc_feature 2935..2975 /label=BBa_B0012 terminator 2935..2975 /label=BBa_B0012 misc_feature 2984..3003 /label=LuxR/HSL promoter 3003..3008 /label=-35 promoter 3025..3030 /label=-10 misc_feature 3036..3036 /label=start misc_feature 2984..3038 /label=BBa_R0062 promoter 2984..3038 /label=BBa_R0062 misc_feature 3051..3054 /label=conserved RBS 3047..3058 /label=BBa_B0034 misc_feature 3644..3675 /label=LVA CDS 3065..3643 /label=luxI misc_feature 3065..3682 /label=BBa_C0061 misc_feature 3683..3707 /label=Help:Barcodes CDS 3065..3682 /label=BBa_C0061 misc_feature 3720..3723 /label=conserved RBS 3716..3727 /label=BBa_B0034 misc_feature 4490..4495 /label=stop misc_feature 4451..4489 /label=AAV misc_feature 3734..4495 /label=BBa_E0034 stem_loop 4515..4558 /label=stem_loop misc_feature 4504..4583 /label=BBa_B0010 terminator 4504..4583 /label=BBa_B0010 stem_loop 4599..4618 /label=T7 TE misc_feature 4625..4625 /label=stop polyA_site 4619..4632 /label=polya misc_feature 4592..4632 /label=BBa_B0012 terminator 4592..4632 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga cagtaaagaa gctttatttc 1261 gtcccagcag gtcgttgtat gttggatcat tcgtctgtta atagtacatt aacaccagga 1321 gaattattag acttaccggt ttggtgttat cttttggaga ctgaagaagg acctatttta 1381 gtagatacag gtatgccaga aagtgcagtt aataatgaag gtctttttaa cggtacattt 1441 gtcgaagggc aggttttacc gaaaatgact gaagaagata gaatcgtgaa tattttaaaa 1501 cgggttggtt atgagccgga agaccttctt tatattatta gttctcactt gcattttgat 1561 catgcaggag gaaatggcgc ttttataaat acaccaatca ttgtacagcg tgctgaatat 1621 gaggcggcgc agcatagcga agaatatttg aaagaatgta tattgccgaa tttaaactac 1681 aaaatcattg aaggtgatta tgaagtcgta ccaggagttc aattattgca tacaccaggc 1741 catactccag ggcatcaatc gctattaatt gagacagaaa aatccggtcc tgtattatta 1801 acgattgatg catcgtatac gaaagagaat tttgaaaatg aagtgccatt tgcgggattt 1861 gattcagaat tagctttatc ttcaattaaa cgtttaaaag aagtggtgat gaaagagaag 1921 ccgattgttt tctttggaca tgatatagag caggaaaggg gatgtaaagt gttccctgaa 1981 tatatagctg caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag 2041 tgtagatcgc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 2101 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 2161 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 2221 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 2281 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 2341 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 2401 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 2461 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 2521 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 2581 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 2641 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 2701 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 2761 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac 2821 gctgctgctg tttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2881 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2941 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacctgta ggatcgtaca 3001 ggtttacgca agaaaatggt ttgttatagt cgaataaata ctagagaaag aggagaaata 3061 ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa 3121 aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt 3181 agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg 3241 tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat 3301 gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat 3361 agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag 3421 tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac 3481 agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc 3541 ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt 3601 gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa 3661 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagaaaga 3721 ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 3781 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 3841 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 3901 gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc 3961 cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 4021 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 4081 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 4141 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 4201 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 4261 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 4321 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg 4381 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 4441 gctgtacaag aggcctgctg caaacgacga aaactacgct gctgctgttt aataatacta 4501 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 4561 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 4621 tctgcgttta ta //