LOCUS       BBa_I13974              2077 bp    DNA     linear       29-Apr-2024
DEFINITION  intermediate for lac synchro device
ACCESSION   BBa_I13974
VERSION     BBa_I13974.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    832..837
                     /label=stop
     misc_feature    793..831
                     /label=AAV
     misc_feature    76..837
                     /label=BBa_E0034
     stem_loop       857..900
                     /label=stem_loop
     misc_feature    846..925
                     /label=BBa_B0010
     terminator      846..925
                     /label=BBa_B0010
     stem_loop       941..960
                     /label=T7 TE
     misc_feature    967..967
                     /label=stop
     polyA_site      961..974
                     /label=polya
     misc_feature    934..974
                     /label=BBa_B0012
     terminator      934..974
                     /label=BBa_B0012
     misc_feature    983..1002
                     /label=LuxR/HSL
     promoter        1097..1104
                     /label=-10
     promoter        1071..1076
                     /label=-35
     misc_feature    1110..1110
                     /label=start
     misc_feature    1112..1131
                     /label=Putative LuxR/HSL
     misc_feature    983..1133
                     /label=BBa_R0063
     promoter        983..1133
                     /label=BBa_R0063
     misc_feature    1146..1149
                     /label=conserved
     RBS             1142..1153
                     /label=BBa_B0034
     misc_feature    1160..1161
                     /label=prefix
     misc_feature    1333..1333
                     /label=T
     misc_feature    1651..1651
                     /label=A
     CDS             1160..1909
                     /label=luxR
     misc_feature    1160..1915
                     /label=BBa_C0062
     misc_feature    1916..1940
                     /label=Help:Barcodes
     CDS             1160..1915
                     /label=BBa_C0062
     stem_loop       1960..2003
                     /label=stem_loop
     misc_feature    1949..2028
                     /label=BBa_B0010
     terminator      1949..2028
                     /label=BBa_B0010
     stem_loop       2044..2063
                     /label=T7 TE
     misc_feature    2070..2070
                     /label=stop
     polyA_site      2064..2077
                     /label=polya
     misc_feature    2037..2077
                     /label=BBa_B0012
     terminator      2037..2077
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac
      301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctgctgctgt ttaataatac
      841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      961 tttctgcgtt tatatactag agacctgtac gatcctacag gtgcttatgt taagtaattg
     1021 tattcccagc gatacaatag tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc
     1081 attaccgttt taatgatata taacacgcaa aacttgcgac aaacaatagg taatactaga
     1141 gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa
     1201 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa
     1261 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc
     1321 tgatatttca atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa
     1381 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg
     1441 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa
     1501 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg
     1561 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc
     1621 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat
     1681 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc
     1741 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt
     1801 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat
     1861 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg
     1921 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1981 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2041 actggctcac cttcgggtgg gcctttctgc gtttata
//