LOCUS BBa_I13977 2083 bp DNA linear 22-Nov-2024 DEFINITION intermediate for lac relaxation oscillator ACCESSION BBa_I13977 VERSION BBa_I13977.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 838..843 /label=stop misc_feature 799..837 /label=AAV misc_feature 82..843 /label=BBa_E0034 stem_loop 863..906 /label=stem_loop misc_feature 852..931 /label=BBa_B0010 terminator 852..931 /label=BBa_B0010 stem_loop 947..966 /label=T7 TE misc_feature 973..973 /label=stop polyA_site 967..980 /label=polya misc_feature 940..980 /label=BBa_B0012 terminator 940..980 /label=BBa_B0012 misc_feature 64..980 /label=BBa_E0434 misc_feature 1..980 /label=BBa_I13976 misc_feature 989..1008 /label=LuxR/HSL promoter 1103..1110 /label=-10 promoter 1077..1082 /label=-35 misc_feature 1116..1116 /label=start misc_feature 1118..1137 /label=Putative LuxR/HSL misc_feature 989..1139 /label=BBa_R0063 promoter 989..1139 /label=BBa_R0063 misc_feature 1152..1155 /label=conserved RBS 1148..1159 /label=BBa_B0034 misc_feature 1166..1167 /label=prefix misc_feature 1339..1339 /label=T misc_feature 1657..1657 /label=A CDS 1166..1915 /label=luxR misc_feature 1166..1921 /label=BBa_C0062 misc_feature 1922..1946 /label=Help:Barcodes CDS 1166..1921 /label=BBa_C0062 stem_loop 1966..2009 /label=stem_loop misc_feature 1955..2034 /label=BBa_B0010 terminator 1955..2034 /label=BBa_B0010 stem_loop 2050..2069 /label=T7 TE misc_feature 2076..2076 /label=stop polyA_site 2070..2083 /label=polya misc_feature 2043..2083 /label=BBa_B0012 terminator 2043..2083 /label=BBa_B0012 misc_feature 989..2083 /label=BBa_S3511 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgctgc tgctgtttaa 841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 961 tgggcctttc tgcgtttata tactagagac ctgtacgatc ctacaggtgc ttatgttaag 1021 taattgtatt cccagcgata caatagtgtg acaaaaatcc aatttattag aatcaaatgt 1081 caatccatta ccgttttaat gatatataac acgcaaaact tgcgacaaac aataggtaat 1141 actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat 1201 aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat 1261 gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt 1321 taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga 1381 cgctaattta ataaaatatg atcctatagt agattattct aactccaatc attcaccaat 1441 taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga 1501 agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg 1561 cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt 1621 acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat 1681 aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc 1741 gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg 1801 tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca 1861 aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata 1921 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 1981 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2041 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata // LOCUS BBa_I13212 1095 bp DNA linear 22-Nov-2024 DEFINITION LuxR Protein Generator Controlled by the Left Lux Promoter ACCESSION BBa_I13212 VERSION BBa_I13212.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttata //