LOCUS BBa_I13990 8555 bp DNA linear 22-Nov-2024 DEFINITION Synchronator 2 w/ CFP under control of Lac (I4202.I13601) ACCESSION BBa_I13990 VERSION BBa_I13990.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 CDS 127..743 /label=tetR misc_feature 744..777 /label=SsrA misc_feature 784..808 /label=Help:Barcodes CDS 124..783 /label=BBa_C0040 stem_loop 828..871 /label=stem_loop misc_feature 817..896 /label=BBa_B0010 terminator 817..896 /label=BBa_B0010 stem_loop 912..931 /label=T7 TE misc_feature 938..938 /label=stop polyA_site 932..945 /label=polya misc_feature 905..945 /label=BBa_B0012 terminator 905..945 /label=BBa_B0012 misc_feature 954..972 /label=TetR 1 misc_feature 954..1007 /label=BBa_R0040 promoter 973..978 /label=-35 misc_feature 979..997 /label=TetR 2 promoter 996..1001 /label=-10 promoter 954..1007 /label=BBa_R0040 misc_feature 1020..1023 /label=conserved RBS 1016..1027 /label=BBa_B0034 misc_feature 2123..2161 /label=LVA CDS 1034..2161 /label=lacI-LVA misc_feature 1034..2161 /label=BBa_C0012 misc_feature 2162..2186 /label=Help:Barcodes CDS 1034..2161 /label=BBa_C0012 stem_loop 2206..2249 /label=stem_loop misc_feature 2195..2274 /label=BBa_B0010 terminator 2195..2274 /label=BBa_B0010 stem_loop 2290..2309 /label=T7 TE misc_feature 2316..2316 /label=stop polyA_site 2310..2323 /label=polya misc_feature 2283..2323 /label=BBa_B0012 terminator 2283..2323 /label=BBa_B0012 CDS 2332..2419 /label=end of LacI coding region (inactive) misc_feature 2332..2531 /label=BBa_R0010 misc_feature 2420..2457 /label=CAP binding site promoter 2468..2473 /label=-35 promoter 2492..2497 /label=-10 misc_feature 2497..2531 /label=LacI binding site misc_feature 2504..2504 /label=start promoter 2332..2531 /label=BBa_R0010 misc_feature 2544..2547 /label=conserved RBS 2540..2551 /label=BBa_B0034 CDS 2561..3268 /label=cI lambda misc_feature 3269..3301 /label=LVA misc_feature 3308..3332 /label=Help:Barcodes CDS 2558..3307 /label=BBa_C0051 stem_loop 3352..3395 /label=stem_loop misc_feature 3341..3420 /label=BBa_B0010 terminator 3341..3420 /label=BBa_B0010 stem_loop 3436..3455 /label=T7 TE misc_feature 3462..3462 /label=stop polyA_site 3456..3469 /label=polya misc_feature 3429..3469 /label=BBa_B0012 terminator 3429..3469 /label=BBa_B0012 promoter 3515..3520 /label=-10 promoter 3492..3497 /label=-35 misc_feature 3502..3518 /label=OR1 misc_feature 3478..3494 /label=OR2 misc_feature 3478..3526 /label=BBa_R0051 promoter 3478..3526 /label=BBa_R0051 misc_feature 3539..3542 /label=conserved RBS 3535..3546 /label=BBa_B0034 misc_feature 4132..4163 /label=LVA CDS 3553..4131 /label=luxI misc_feature 3553..4170 /label=BBa_C0061 misc_feature 4171..4195 /label=Help:Barcodes CDS 3553..4170 /label=BBa_C0061 stem_loop 4215..4258 /label=stem_loop misc_feature 4204..4283 /label=BBa_B0010 terminator 4204..4283 /label=BBa_B0010 stem_loop 4299..4318 /label=T7 TE misc_feature 4325..4325 /label=stop polyA_site 4319..4332 /label=polya misc_feature 4292..4332 /label=BBa_B0012 terminator 4292..4332 /label=BBa_B0012 misc_feature 4341..4360 /label=LuxR/HSL promoter 4455..4462 /label=-10 promoter 4429..4434 /label=-35 misc_feature 4468..4468 /label=start misc_feature 4470..4489 /label=Putative LuxR/HSL misc_feature 4341..4491 /label=BBa_R0063 promoter 4341..4491 /label=BBa_R0063 misc_feature 4504..4507 /label=conserved RBS 4500..4511 /label=BBa_B0034 misc_feature 4518..4519 /label=prefix misc_feature 4691..4691 /label=T misc_feature 5009..5009 /label=A CDS 4518..5267 /label=luxR misc_feature 4518..5273 /label=BBa_C0062 misc_feature 5274..5298 /label=Help:Barcodes CDS 4518..5273 /label=BBa_C0062 stem_loop 5318..5361 /label=stem_loop misc_feature 5307..5386 /label=BBa_B0010 terminator 5307..5386 /label=BBa_B0010 stem_loop 5402..5421 /label=T7 TE misc_feature 5428..5428 /label=stop polyA_site 5422..5435 /label=polya misc_feature 5395..5435 /label=BBa_B0012 terminator 5395..5435 /label=BBa_B0012 CDS 5444..5531 /label=end of LacI coding region (inactive) misc_feature 5444..5643 /label=BBa_R0010 misc_feature 5532..5569 /label=CAP binding site promoter 5580..5585 /label=-35 promoter 5604..5609 /label=-10 misc_feature 5609..5643 /label=LacI binding site misc_feature 5616..5616 /label=start promoter 5444..5643 /label=BBa_R0010 misc_feature 5656..5659 /label=conserved RBS 5652..5663 /label=BBa_B0034 misc_feature 5670..5672 /label=start misc_feature 6453..6458 /label=2 misc_feature 6420..6452 /label=LVA CDS 5670..6419 /label=aiiA misc_feature 5670..6458 /label=BBa_C0060 misc_feature 6459..6483 /label=Help:Barcodes CDS 5670..6458 /label=BBa_C0060 stem_loop 6503..6546 /label=stem_loop misc_feature 6492..6571 /label=BBa_B0010 terminator 6492..6571 /label=BBa_B0010 stem_loop 6587..6606 /label=T7 TE misc_feature 6613..6613 /label=stop polyA_site 6607..6620 /label=polya misc_feature 6580..6620 /label=BBa_B0012 terminator 6580..6620 /label=BBa_B0012 misc_feature 6629..6647 /label=TetR 1 misc_feature 6629..6682 /label=BBa_R0040 promoter 6648..6653 /label=-35 misc_feature 6654..6672 /label=TetR 2 promoter 6671..6676 /label=-10 promoter 6629..6682 /label=BBa_R0040 misc_feature 6695..6698 /label=conserved RBS 6691..6702 /label=BBa_B0034 CDS 6709..7470 /label=YFP (LVA) misc_feature 7465..7470 /label=2 misc_feature 7426..7464 /label=SsrA misc_feature 6950..6950 /label=A (Q->L) misc_feature 6709..7470 /label=BBa_E0032 misc_feature 6709..7470 /label=BBa_E0032 stem_loop 7490..7533 /label=stem_loop misc_feature 7479..7558 /label=BBa_B0010 terminator 7479..7558 /label=BBa_B0010 stem_loop 7574..7593 /label=T7 TE misc_feature 7600..7600 /label=stop polyA_site 7594..7607 /label=polya misc_feature 7567..7607 /label=BBa_B0012 terminator 7567..7607 /label=BBa_B0012 misc_feature 7618..7634 /label=lac O1 promoter 7635..7640 /label=-35 misc_feature 7641..7657 /label=lac O1 promoter 7658..7663 /label=-10 misc_feature 7616..7669 /label=BBa_R0011 promoter 7616..7669 /label=BBa_R0011 misc_feature 7682..7685 /label=conserved RBS 7678..7689 /label=BBa_B0034 CDS 7696..8418 /label=BBa_E0020 stem_loop 8438..8481 /label=stem_loop misc_feature 8427..8506 /label=BBa_B0010 terminator 8427..8506 /label=BBa_B0010 stem_loop 8522..8541 /label=T7 TE misc_feature 8548..8548 /label=stop polyA_site 8542..8555 /label=polya misc_feature 8515..8555 /label=BBa_B0012 terminator 8515..8555 /label=BBa_B0012 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag 181 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct 241 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg 301 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta 361 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta 421 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt 481 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat 541 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa 601 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac 661 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa 721 caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa 781 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat 961 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga 1021 ggagaaatac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 1081 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 1141 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 1201 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 1261 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 1321 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 1381 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 1441 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 1501 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 1561 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 1621 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 1681 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 1741 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 1801 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata 1861 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 1921 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 1981 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 2041 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 2101 ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata 2161 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct 2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2281 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gcaatacgca 2341 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 2401 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 2461 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 2521 aatttcacac atactagaga aagaggagaa atactagatg agcacaaaaa agaaaccatt 2581 aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa 2641 tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt 2701 tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa 2761 aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat 2821 gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc 2881 tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga 2941 gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg 3001 taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct 3061 cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga 3121 tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa 3181 cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc 3241 tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg ctttagtagc 3301 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga 3361 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 3421 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtaa 3481 caccgtgcgt gttgactatt ttacctctgg cggtgataat ggttgctact agagaaagag 3541 gagaaatact agatgactat aatgataaaa aaatcggatt ttttggcaat tccatcggag 3601 gagtataaag gtattctaag tcttcgttat caagtgttta agcaaagact tgagtgggac 3661 ttagttgtag aaaataacct tgaatcagat gagtatgata actcaaatgc agaatatatt 3721 tatgcttgtg atgatactga aaatgtaagt ggatgctggc gtttattacc tacaacaggt 3781 gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat 3841 cctaatatag tcgaattaag tcgttttgct gtaggtaaaa atagctcaaa gataaataac 3901 tctgctagtg aaattacaat gaaactattt gaagctatat ataaacacgc tgttagtcaa 3961 ggtattacag aatatgtaac agtaacatca acagcaatag agcgattttt aaagcgtatt 4021 aaagttcctt gtcatcgtat tggagacaaa gaaattcatg tattaggtga tactaaatcg 4081 gttgtattgt ctatgcctat taatgaacag tttaaaaaag cagtcttaaa tgctgcaaac 4141 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta 4201 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 4261 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 4321 tctgcgttta tatactagag acctgtacga tcctacaggt gcttatgtta agtaattgta 4381 ttcccagcga tacaatagtg tgacaaaaat ccaatttatt agaatcaaat gtcaatccat 4441 taccgtttta atgatatata acacgcaaaa cttgcgacaa acaataggta atactagaga 4501 aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga ataattaata 4561 aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa 4621 tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg 4681 atatttcaat cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt 4741 taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga 4801 atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa 4861 catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa 4921 tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt 4981 gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag 5041 caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat 5101 gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca 5161 ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt 5221 ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat 5281 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 5341 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 5401 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcaatacg caaaccgcct 5461 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 5521 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 5581 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 5641 acatactaga gaaagaggag aaatactaga tgacagtaaa gaagctttat ttcgtcccag 5701 caggtcgttg tatgttggat cattcgtctg ttaatagtac attaacacca ggagaattat 5761 tagacttacc ggtttggtgt tatcttttgg agactgaaga aggacctatt ttagtagata 5821 caggtatgcc agaaagtgca gttaataatg aaggtctttt taacggtaca tttgtcgaag 5881 ggcaggtttt accgaaaatg actgaagaag atagaatcgt gaatatttta aaacgggttg 5941 gttatgagcc ggaagacctt ctttatatta ttagttctca cttgcatttt gatcatgcag 6001 gaggaaatgg cgcttttata aatacaccaa tcattgtaca gcgtgctgaa tatgaggcgg 6061 cgcagcatag cgaagaatat ttgaaagaat gtatattgcc gaatttaaac tacaaaatca 6121 ttgaaggtga ttatgaagtc gtaccaggag ttcaattatt gcatacacca ggccatactc 6181 cagggcatca atcgctatta attgagacag aaaaatccgg tcctgtatta ttaacgattg 6241 atgcatcgta tacgaaagag aattttgaaa atgaagtgcc atttgcggga tttgattcag 6301 aattagcttt atcttcaatt aaacgtttaa aagaagtggt gatgaaagag aagccgattg 6361 ttttctttgg acatgatata gagcaggaaa ggggatgtaa agtgttccct gaatatatag 6421 ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat 6481 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 6541 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 6601 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat 6661 cagtgataga gatactgagc actactagag aaagaggaga aatactagat ggtgagcaag 6721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 6781 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 6841 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 6901 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc 6961 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 7021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 7081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 7141 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 7201 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 7261 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 7321 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 7381 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac 7441 gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa 7501 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 7561 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaattg 7621 tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcact actagagaaa 7681 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 7741 ctggtcgagc tggacggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgag 7801 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 7861 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac 7921 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 7981 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 8041 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 8101 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc 8161 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc 8221 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 8281 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag 8341 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 8401 gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 8461 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 8521 tggctcacct tcgggtgggc ctttctgcgt ttata //