##gff-version 3
##sequence-region BBa_I14013 1 948
BBa_I14013	.	ribosome_entry_site	166	180	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I14013	.	ribosome_entry_site	173	177	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I14013	.	ribosome_entry_site	166	180	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I14013	.	engineered_region	166	180	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I14013	.	promoter	1	157	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_I14013	.	non_covalent_binding_site	106	125	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_I14013	.	promoter	117	122	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_I14013	.	promoter	140	145	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_I14013	.	non_covalent_binding_site	46	63	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_I14013	.	engineered_region	187	948	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I14013	.	stop_codon	943	948	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I14013	.	sequence_alteration	428	428	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I14013	.	engineered_region	187	948	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I14013	.	CDS	187	948	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I14013	.	tag	904	942	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
>BBa_I14013
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagct
actagagattaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtc
gagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctga
ccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgca
atgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggag
cgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaacc
gcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagcca
caacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggc
agcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacc
tgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgc
cgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa