LOCUS       BBa_I14014              1010 bp    DNA     linear       29-Apr-2024
DEFINITION  R10, B30, C79
ACCESSION   BBa_I14014
VERSION     BBa_I14014.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..946
                     /label=lasR
     misc_feature    947..979
                     /label=LVA
     misc_feature    709..709
                     /label=G
     misc_feature    986..1010
                     /label=Help:Barcodes
     CDS             230..985
                     /label=BBa_C0079
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggccttggt
      241 tgacggtttt cttgagctgg aacgctcaag tggaaaattg gagtggagcg ccatcctcca
      301 gaagatggcg agcgaccttg gattctcgaa gatcctgttc ggcctgttgc ctaaggacag
      361 ccaggactac gagaacgcct tcatcgtcgg caactacccg gccgcctggc gcgagcatta
      421 cgaccgggct ggctacgcgc gggtcgaccc gacggtcagt cactgtaccc agagcgtact
      481 gccgattttc tgggaaccgt ccatctacca gacgcgaaag cagcacgagt tcttcgagga
      541 agcctcggcc gccggcctgg tgtatgggct gaccatgccg ctgcatggtg ctcgcggcga
      601 actcggcgcg ctgagcctca gcgtggaagc ggaaaaccgg gccgaggcca accgtttcat
      661 agagtcggtc ctgccgaccc tgtggatgct caaggactac gcactgcaaa gcggtgccgg
      721 actggccttc gaacatccgg tcagcaaacc ggtggttctg accagccggg agaaggaagt
      781 gttgcagtgg tgcgccatcg gcaagaccag ttgggagata tcggttatct gcaactgctc
      841 ggaagccaat gtgaacttcc atatgggaaa tattcggcgg aagttcggtg tgacctcccg
      901 ccgcgtagcg gccattatgg ccgttaattt gggtcttatt actctcgctg caaacgacga
      961 aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
//