BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_I14024
1
BBa_I14024
Constitutive RhlR
2004-08-01T11:00:00Z
2015-08-31T04:07:37Z
p(Bla),
Strong RBS,
RhlR + LVA
false
false
_4_
0
171
7
Not in stock
false
false
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
component1028598
1
BBa_I14018
component1028606
1
BBa_B0030
component1028617
1
BBa_C0071
annotation1028598
1
BBa_I14018
range1028598
1
1
35
annotation1028606
1
BBa_B0030
range1028606
1
44
58
annotation1028617
1
BBa_C0071
range1028617
1
65
826
BBa_I14018
1
Bla
P(Bla)
2004-08-01T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pUC19
Released HQ 2013
P(Bla), Medium Transcription
false
false
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1027016
1
-10
range1027016
1
25
30
annotation1027020
1
-35
range1027020
1
4
9
annotation1018027
1
P(Bla)
range1018027
1
1
35
BBa_C0071
1
rhlR
rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
2004-01-26T12:00:00Z
2015-08-31T04:07:23Z
P. aeruginosa PA3477
Released HQ 2013
Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
false
false
_1_
0
24
7
In stock
false
(1/28/04 Edit Jamboree)
(0) No BB sites
(1) ATG
(2) TAATAA
(3) Blast checks out
(4) No codon changes
(5) Maybe chassis compatability (check by experiment)
(6) Codon usage not optimal (but looks OK)
(7) ssrA tag added
true
Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
annotation306523
1
LVA
range306523
1
724
756
annotation2213995
1
Help:Barcodes
range2213995
1
763
787
annotation301192
1
rhlR
range301192
1
1
723
BBa_I14024_sequence
1
tgtaagtttatacataggcgagtactctgttatggtactagagattaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_I14018_sequence
1
tgtaagtttatacataggcgagtactctgttatgg
BBa_C0071_sequence
1
atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z