LOCUS       BBa_I14029              1705 bp    DNA     linear       02-May-2024
DEFINITION  Lac Toggle (strong RBS)
ACCESSION   BBa_I14029
VERSION     BBa_I14029.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             233..849
                     /label=tetR
     misc_feature    850..883
                     /label=SsrA
     misc_feature    890..914
                     /label=Help:Barcodes
     CDS             230..889
                     /label=BBa_C0040
     RBS             923..937
                     /label=RBS-1\Strong
     RBS             930..934
                     /label=RBS
     misc_feature    923..937
                     /label=BBa_B0030
     RBS             923..937
                     /label=BBa_B0030
     CDS             944..1705
                     /label=CFP (LVA)
     misc_feature    1662..1699
                     /label=SsrA
     misc_feature    1700..1705
                     /label=2
     misc_feature    1012..1012
                     /label=A
     misc_feature    944..1705
                     /label=BBa_E0022
     misc_feature    944..1705
                     /label=BBa_E0022
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgtccagatt
      241 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
      301 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca
      361 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca
      421 tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa
      481 aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg
      541 gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg
      601 tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg
      661 cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga
      721 tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc
      781 agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga
      841 aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg
      901 ctagtgtaga tcactactag agattaaaga ggagaaatac tagatggtga gcaagggcga
      961 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg tgaacggcca
     1021 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1081 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1141 ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1201 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1261 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1321 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1381 catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     1441 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1501 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1561 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1621 cgccgccggg atcactctcg gcatggacga gctgtacaag aggcctgctg caaacgacga
     1681 aaactacgct ttagtagctt aataa
//