LOCUS       BBa_I14030              2830 bp    DNA     linear       29-Apr-2024
DEFINITION  Tet Toggle (strong RBS, weak RBS)
ACCESSION   BBa_I14030
VERSION     BBa_I14030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    1173..1211
                     /label=LVA
     CDS             84..1211
                     /label=lacI-LVA
     misc_feature    84..1211
                     /label=BBa_C0012
     misc_feature    1212..1236
                     /label=Help:Barcodes
     CDS             84..1211
                     /label=BBa_C0012
     misc_feature    1251..1254
                     /label=conserved
     RBS             1245..1258
                     /label=BBa_B0031
     CDS             1268..1975
                     /label=cI lambda
     misc_feature    1976..2008
                     /label=LVA
     misc_feature    2015..2039
                     /label=Help:Barcodes
     CDS             1265..2014
                     /label=BBa_C0051
     RBS             2048..2062
                     /label=RBS-1\Strong
     RBS             2055..2059
                     /label=RBS
     misc_feature    2048..2062
                     /label=BBa_B0030
     RBS             2048..2062
                     /label=BBa_B0030
     CDS             2069..2830
                     /label=YFP (LVA)
     misc_feature    2825..2830
                     /label=2
     misc_feature    2786..2824
                     /label=SsrA
     misc_feature    2310..2310
                     /label=A (Q->L)
     misc_feature    2069..2830
                     /label=BBa_E0032
     misc_feature    2069..2830
                     /label=BBa_E0032
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg
      121 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
      181 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
      241 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
      301 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
      361 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
      421 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
      481 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
      541 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
      601 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
      661 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
      721 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
      781 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
      841 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
      901 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
      961 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     1021 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     1081 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1141 cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag
     1201 tagcttaata actctgatag tgctagtgta gatctctact agagtcacac aggaaaccta
     1261 ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct
     1321 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga
     1381 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa
     1441 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc
     1501 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag
     1561 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct
     1621 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga
     1681 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc
     1741 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga
     1801 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag
     1861 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag
     1921 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc
     1981 aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct
     2041 actagagatt aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg
     2101 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     2161 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     2221 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg
     2281 cttcgcccgc taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga
     2341 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     2401 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     2461 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     2521 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     2581 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     2641 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     2701 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     2761 tctcggcatg gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt
     2821 agcttaataa
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