LOCUS       BBa_I14048               675 bp    DNA     linear       25-Nov-2024
DEFINITION  PenI toggle
ACCESSION   BBa_I14048
VERSION     BBa_I14048.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..614
                     /label=penI
     misc_feature    612..644
                     /label=LVA
     misc_feature    651..675
                     /label=Help:Barcodes
     CDS             228..650
                     /label=BBa_C0074
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg aaaaaaatac
      241 cacagatttc tgatgcggaa ctcgaagtca tgaaagtgat ttggaagcat tcttccatta
      301 atactaatga ggtaatcaaa gagctcagta aaacttcaac gtggagccca aaaactattc
      361 agactatgct gctgcgcctt atcaaaaaag gcgctctcaa ccaccataaa gaaggtcggg
      421 ttttcgttta cacgcctaat atagacgaat cagattatat agaggttaag tcacactcat
      481 ttctcaaccg gttttacaat ggtacattaa attccatggt actcaacttt ttggagaatg
      541 atcaactgtc gggagaagaa atcaatgaat tgtatcagat actcgaagaa cataagaacc
      601 gtaagaagga agctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
      661 gctagtgtag atctc
//