LOCUS       BBa_I14054              1703 bp    DNA     linear       04-May-2024
DEFINITION  Lac Toggle (medium RBS)
ACCESSION   BBa_I14054
VERSION     BBa_I14054.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             231..847
                     /label=tetR
     misc_feature    848..881
                     /label=SsrA
     misc_feature    888..912
                     /label=Help:Barcodes
     CDS             228..887
                     /label=BBa_C0040
     RBS             921..935
                     /label=RBS-1\Strong
     RBS             928..932
                     /label=RBS
     misc_feature    921..935
                     /label=BBa_B0030
     RBS             921..935
                     /label=BBa_B0030
     CDS             942..1703
                     /label=CFP (LVA)
     misc_feature    1660..1697
                     /label=SsrA
     misc_feature    1698..1703
                     /label=2
     misc_feature    1010..1010
                     /label=A
     misc_feature    942..1703
                     /label=BBa_E0022
     misc_feature    942..1703
                     /label=BBa_E0022
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg tccagattag
      241 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
      301 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
      361 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
      421 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
      481 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
      541 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
      601 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
      661 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
      721 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
      781 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
      841 gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct
      901 agtgtagatc actactagag attaaagagg agaaatacta gatggtgagc aagggcgagg
      961 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgtg aacggccaca
     1021 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1081 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1141 ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1201 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1261 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1321 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1381 tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     1441 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1501 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1561 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1621 ccgccgggat cactctcggc atggacgagc tgtacaagag gcctgctgca aacgacgaaa
     1681 actacgcttt agtagcttaa taa
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