LOCUS       BBa_I14055              2828 bp    DNA     linear       04-May-2024
DEFINITION  Tet Toggle (medium RBS, weak RBS)
ACCESSION   BBa_I14055
VERSION     BBa_I14055.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    1171..1209
                     /label=LVA
     CDS             82..1209
                     /label=lacI-LVA
     misc_feature    82..1209
                     /label=BBa_C0012
     misc_feature    1210..1234
                     /label=Help:Barcodes
     CDS             82..1209
                     /label=BBa_C0012
     misc_feature    1249..1252
                     /label=conserved
     RBS             1243..1256
                     /label=BBa_B0031
     CDS             1266..1973
                     /label=cI lambda
     misc_feature    1974..2006
                     /label=LVA
     misc_feature    2013..2037
                     /label=Help:Barcodes
     CDS             1263..2012
                     /label=BBa_C0051
     RBS             2046..2060
                     /label=RBS-1\Strong
     RBS             2053..2057
                     /label=RBS
     misc_feature    2046..2060
                     /label=BBa_B0030
     RBS             2046..2060
                     /label=BBa_B0030
     CDS             2067..2828
                     /label=YFP (LVA)
     misc_feature    2823..2828
                     /label=2
     misc_feature    2784..2822
                     /label=SsrA
     misc_feature    2308..2308
                     /label=A (Q->L)
     misc_feature    2067..2828
                     /label=BBa_E0032
     misc_feature    2067..2828
                     /label=BBa_E0032
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca
      121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
      181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
      241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
      301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
      361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
      421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
      481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
      541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
      601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
      661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
      721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
      781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
      841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
      901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
      961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
     1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1141 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta
     1201 gcttaataac tctgatagtg ctagtgtaga tctctactag agtcacacag gaaacctact
     1261 agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta
     1321 aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca
     1381 agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg
     1441 cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa tttagccctt
     1501 caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa
     1561 gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta
     1621 gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt
     1681 ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa
     1741 gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt
     1801 tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg
     1861 gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt
     1921 gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt ggcgctgcaa
     1981 acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt agatcgctac
     2041 tagagattaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg
     2101 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     2161 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     2221 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct
     2281 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag
     2341 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     2401 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2461 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     2521 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     2581 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2641 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     2701 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2761 tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctttagtag
     2821 cttaataa
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