LOCUS       BBa_I14057              2825 bp    DNA     linear       03-May-2024
DEFINITION  Tet Toggle (medium RBS, weaker RBS)
ACCESSION   BBa_I14057
VERSION     BBa_I14057.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    1171..1209
                     /label=LVA
     CDS             82..1209
                     /label=lacI-LVA
     misc_feature    82..1209
                     /label=BBa_C0012
     misc_feature    1210..1234
                     /label=Help:Barcodes
     CDS             82..1209
                     /label=BBa_C0012
     RBS             1243..1253
                     /label=RBS-4\Weaker
     RBS             1249..1252
                     /label=RBS
     misc_feature    1243..1253
                     /label=BBa_B0033
     RBS             1243..1253
                     /label=BBa_B0033
     CDS             1263..1970
                     /label=cI lambda
     misc_feature    1971..2003
                     /label=LVA
     misc_feature    2010..2034
                     /label=Help:Barcodes
     CDS             1260..2009
                     /label=BBa_C0051
     RBS             2043..2057
                     /label=RBS-1\Strong
     RBS             2050..2054
                     /label=RBS
     misc_feature    2043..2057
                     /label=BBa_B0030
     RBS             2043..2057
                     /label=BBa_B0030
     CDS             2064..2825
                     /label=YFP (LVA)
     misc_feature    2820..2825
                     /label=2
     misc_feature    2781..2819
                     /label=SsrA
     misc_feature    2305..2305
                     /label=A (Q->L)
     misc_feature    2064..2825
                     /label=BBa_E0032
     misc_feature    2064..2825
                     /label=BBa_E0032
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca
      121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
      181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
      241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
      301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
      361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
      421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
      481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
      541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
      601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
      661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
      721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
      781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
      841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
      901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
      961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
     1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1141 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta
     1201 gcttaataac tctgatagtg ctagtgtaga tctctactag agtcacacag gactactaga
     1261 tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag
     1321 caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga
     1381 tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt
     1441 ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa
     1501 tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg
     1561 agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa
     1621 cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg
     1681 cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct
     1741 ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct
     1801 gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc
     1861 aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt
     1921 ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gctgcaaacg
     1981 acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag
     2041 agattaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg
     2101 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2161 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2221 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg
     2281 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct
     2341 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2401 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2461 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2521 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2581 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2641 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     2701 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2761 gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct ttagtagctt
     2821 aataa
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