LOCUS       BBa_I14103              1598 bp    DNA     linear       27-Dec-2024
DEFINITION  penI inverter + tetR inverter
ACCESSION   BBa_I14103
VERSION     BBa_I14103.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..405
                     /label=penI
     misc_feature    403..435
                     /label=LVA
     misc_feature    442..466
                     /label=Help:Barcodes
     CDS             19..441
                     /label=BBa_C0074
     stem_loop       486..529
                     /label=stem_loop
     misc_feature    475..554
                     /label=BBa_B0010
     terminator      475..554
                     /label=BBa_B0010
     stem_loop       570..589
                     /label=T7 TE
     misc_feature    596..596
                     /label=stop
     polyA_site      590..603
                     /label=polya
     misc_feature    563..603
                     /label=BBa_B0012
     terminator      563..603
                     /label=BBa_B0012
     misc_feature    612..688
                     /label=PenI
     promoter        634..639
                     /label=-35
     promoter        657..662
                     /label=-10
     misc_feature    642..664
                     /label=dimer left half
     misc_feature    666..687
                     /label=dimer right half
     promoter        612..688
                     /label=BBa_R0074
     misc_feature    1..688
                     /label=BBa_Q04740
     RBS             697..708
                     /label=BBa_B0064
     CDS             718..1334
                     /label=tetR
     misc_feature    1335..1368
                     /label=SsrA
     misc_feature    1375..1399
                     /label=Help:Barcodes
     CDS             715..1399
                     /label=BBa_C0040
     stem_loop       1419..1462
                     /label=stem_loop
     misc_feature    1408..1487
                     /label=BBa_B0010
     terminator      1408..1487
                     /label=BBa_B0010
     stem_loop       1503..1522
                     /label=T7 TE
     misc_feature    1529..1529
                     /label=stop
     polyA_site      1523..1536
                     /label=polya
     misc_feature    1496..1536
                     /label=BBa_B0012
     terminator      1496..1536
                     /label=BBa_B0012
     terminator      1408..1536
                     /label=BBa_B0015
     misc_feature    1545..1563
                     /label=TetR 1
     misc_feature    1545..1598
                     /label=BBa_R0040
     promoter        1564..1569
                     /label=-35
     misc_feature    1570..1588
                     /label=TetR 2
     promoter        1587..1592
                     /label=-10
     promoter        1545..1598
                     /label=BBa_R0040
     misc_feature    697..1598
                     /label=BBa_Q04401
ORIGIN
        1 aaagaggaga aatactagat gaaaaaaata ccacagattt ctgatgcgga actcgaagtc
       61 atgaaagtga tttggaagca ttcttccatt aatactaatg aggtaatcaa agagctcagt
      121 aaaacttcaa cgtggagccc aaaaactatt cagactatgc tgctgcgcct tatcaaaaaa
      181 ggcgctctca accaccataa agaaggtcgg gttttcgttt acacgcctaa tatagacgaa
      241 tcagattata tagaggttaa gtcacactca tttctcaacc ggttttacaa tggtacatta
      301 aattccatgg tactcaactt tttggagaat gatcaactgt cgggagaaga aatcaatgaa
      361 ttgtatcaga tactcgaaga acataagaac cgtaagaagg aagctgcaaa cgacgaaaac
      421 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagccaggc
      481 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      541 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      601 atatactaga gtcatttcca accgaaaaaa ggcttgcatt taaatcttac atatgtaata
      661 ctttcaaaga ctacatttgt aagatttgta ctagagaaag aggggaaata ctagatgtcc
      721 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc
      781 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat
      841 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg
      901 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac
      961 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt
     1021 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa
     1081 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta
     1141 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact
     1201 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca
     1261 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa
     1321 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga
     1381 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1441 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1501 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag
     1561 agattgacat ccctatcagt gatagagata ctgagcac
//