##gff-version 3
##sequence-region BBa_I14103 1 1598
BBa_I14103	.	engineered_region	1	688	.	+	0	ID=BBa_Q04740;Name=BBa_Q04740
BBa_I14103	.	terminator	475	554	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04740
BBa_I14103	.	engineered_region	475	554	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I14103	.	stem_loop	486	529	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I14103	.	CDS	19	441	.	+	0	ID=BBa_C0074;Name=BBa_C0074;Parent=BBa_Q04740
BBa_I14103	.	tag	403	435	.	+	0	ID=annotation306563;Name=LVA;Parent=BBa_C0074
BBa_I14103	.	engineered_tag	442	466	.	+	0	ID=annotation2214009;Name=Help:Barcodes;Parent=BBa_C0074
BBa_I14103	.	CDS	19	405	.	+	0	ID=annotation302638;Name=penI;Parent=BBa_C0074
BBa_I14103	.	promoter	612	688	.	+	0	ID=BBa_R0074;Name=BBa_R0074;Parent=BBa_Q04740
BBa_I14103	.	non_covalent_binding_site	642	664	.	+	0	ID=annotation319788;Name=dimer left half;Parent=BBa_R0074
BBa_I14103	.	promoter	634	639	.	+	0	ID=annotation319211;Name=-35;Parent=BBa_R0074
BBa_I14103	.	operator	612	688	.	+	0	ID=annotation302713;Name=PenI;Parent=BBa_R0074
BBa_I14103	.	non_covalent_binding_site	666	687	.	+	0	ID=annotation319789;Name=dimer right half;Parent=BBa_R0074
BBa_I14103	.	promoter	657	662	.	+	0	ID=annotation319212;Name=-10;Parent=BBa_R0074
BBa_I14103	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04740
BBa_I14103	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I14103	.	terminator	563	603	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04740
BBa_I14103	.	stem_loop	570	589	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I14103	.	engineered_region	563	603	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I14103	.	stop_codon	596	596	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I14103	.	polyA_site	590	603	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I14103	.	engineered_region	697	1598	.	+	0	ID=BBa_Q04401;Name=BBa_Q04401
BBa_I14103	.	promoter	1545	1598	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04401
BBa_I14103	.	promoter	1564	1569	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I14103	.	promoter	1587	1592	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I14103	.	non_covalent_binding_site	1570	1588	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I14103	.	non_covalent_binding_site	1545	1563	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I14103	.	engineered_region	1545	1598	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I14103	.	CDS	715	1399	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04401
BBa_I14103	.	engineered_tag	1375	1399	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I14103	.	CDS	718	1334	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I14103	.	tag	1335	1368	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I14103	.	terminator	1408	1536	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04401
BBa_I14103	.	terminator	1496	1536	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I14103	.	stem_loop	1503	1522	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I14103	.	engineered_region	1496	1536	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I14103	.	stop_codon	1529	1529	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I14103	.	polyA_site	1523	1536	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I14103	.	terminator	1408	1487	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I14103	.	engineered_region	1408	1487	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I14103	.	stem_loop	1419	1462	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I14103	.	ribosome_entry_site	697	708	.	+	0	ID=BBa_B0064;Name=BBa_B0064;Parent=BBa_Q04401
>BBa_I14103
aaagaggagaaatactagatgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagc
attcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactat
gctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagac
gaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactca
actttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaa
gaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcatttccaaccgaaaaaagg
cttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttgtactagagaaagaggggaaatac
tagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaa
caacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgct
cgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttt
ttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggc
ctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatt
atatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaa
agggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagc
cagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaa
cgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagt
gatagagatactgagcac