LOCUS       BBa_I14104              1598 bp    DNA     linear       22-Dec-2024
DEFINITION  tetR + penI inverters in series
ACCESSION   BBa_I14104
VERSION     BBa_I14104.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=BBa_B0064
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..703
                     /label=BBa_C0040
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     terminator      712..840
                     /label=BBa_B0015
     misc_feature    849..867
                     /label=TetR 1
     misc_feature    849..902
                     /label=BBa_R0040
     promoter        868..873
                     /label=-35
     misc_feature    874..892
                     /label=TetR 2
     promoter        891..896
                     /label=-10
     promoter        849..902
                     /label=BBa_R0040
     misc_feature    1..902
                     /label=BBa_Q04401
     misc_feature    915..918
                     /label=conserved
     RBS             911..922
                     /label=BBa_B0034
     CDS             929..1315
                     /label=penI
     misc_feature    1313..1345
                     /label=LVA
     misc_feature    1352..1376
                     /label=Help:Barcodes
     CDS             929..1351
                     /label=BBa_C0074
     stem_loop       1396..1439
                     /label=stem_loop
     misc_feature    1385..1464
                     /label=BBa_B0010
     terminator      1385..1464
                     /label=BBa_B0010
     stem_loop       1480..1499
                     /label=T7 TE
     misc_feature    1506..1506
                     /label=stop
     polyA_site      1500..1513
                     /label=polya
     misc_feature    1473..1513
                     /label=BBa_B0012
     terminator      1473..1513
                     /label=BBa_B0012
     misc_feature    1522..1598
                     /label=PenI
     promoter        1544..1549
                     /label=-35
     promoter        1567..1572
                     /label=-10
     misc_feature    1552..1574
                     /label=dimer left half
     misc_feature    1576..1597
                     /label=dimer right half
     promoter        1522..1598
                     /label=BBa_R0074
     misc_feature    911..1598
                     /label=BBa_Q04740
ORIGIN
        1 aaagagggga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      901 actactagag aaagaggaga aatactagat gaaaaaaata ccacagattt ctgatgcgga
      961 actcgaagtc atgaaagtga tttggaagca ttcttccatt aatactaatg aggtaatcaa
     1021 agagctcagt aaaacttcaa cgtggagccc aaaaactatt cagactatgc tgctgcgcct
     1081 tatcaaaaaa ggcgctctca accaccataa agaaggtcgg gttttcgttt acacgcctaa
     1141 tatagacgaa tcagattata tagaggttaa gtcacactca tttctcaacc ggttttacaa
     1201 tggtacatta aattccatgg tactcaactt tttggagaat gatcaactgt cgggagaaga
     1261 aatcaatgaa ttgtatcaga tactcgaaga acataagaac cgtaagaagg aagctgcaaa
     1321 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact
     1381 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1441 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1501 ttctgcgttt atatactaga gtcatttcca accgaaaaaa ggcttgcatt taaatcttac
     1561 atatgtaata ctttcaaaga ctacatttgt aagatttg
//