##gff-version 3
##sequence-region BBa_I14104 1 1598
BBa_I14104	.	engineered_region	1	902	.	+	0	ID=BBa_Q04401;Name=BBa_Q04401
BBa_I14104	.	CDS	19	703	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04401
BBa_I14104	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I14104	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I14104	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I14104	.	promoter	849	902	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04401
BBa_I14104	.	promoter	868	873	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I14104	.	non_covalent_binding_site	874	892	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I14104	.	engineered_region	849	902	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I14104	.	non_covalent_binding_site	849	867	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I14104	.	promoter	891	896	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I14104	.	terminator	712	840	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04401
BBa_I14104	.	terminator	712	791	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I14104	.	stem_loop	723	766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I14104	.	engineered_region	712	791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I14104	.	terminator	800	840	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I14104	.	engineered_region	800	840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I14104	.	stop_codon	833	833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I14104	.	stem_loop	807	826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I14104	.	polyA_site	827	840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I14104	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0064;Name=BBa_B0064;Parent=BBa_Q04401
BBa_I14104	.	engineered_region	911	1598	.	+	0	ID=BBa_Q04740;Name=BBa_Q04740
BBa_I14104	.	ribosome_entry_site	911	922	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04740
BBa_I14104	.	conserved_region	915	918	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I14104	.	CDS	929	1351	.	+	0	ID=BBa_C0074;Name=BBa_C0074;Parent=BBa_Q04740
BBa_I14104	.	CDS	929	1315	.	+	0	ID=annotation302638;Name=penI;Parent=BBa_C0074
BBa_I14104	.	engineered_tag	1352	1376	.	+	0	ID=annotation2214009;Name=Help:Barcodes;Parent=BBa_C0074
BBa_I14104	.	tag	1313	1345	.	+	0	ID=annotation306563;Name=LVA;Parent=BBa_C0074
BBa_I14104	.	promoter	1522	1598	.	+	0	ID=BBa_R0074;Name=BBa_R0074;Parent=BBa_Q04740
BBa_I14104	.	promoter	1544	1549	.	+	0	ID=annotation319211;Name=-35;Parent=BBa_R0074
BBa_I14104	.	non_covalent_binding_site	1552	1574	.	+	0	ID=annotation319788;Name=dimer left half;Parent=BBa_R0074
BBa_I14104	.	promoter	1567	1572	.	+	0	ID=annotation319212;Name=-10;Parent=BBa_R0074
BBa_I14104	.	non_covalent_binding_site	1576	1597	.	+	0	ID=annotation319789;Name=dimer right half;Parent=BBa_R0074
BBa_I14104	.	operator	1522	1598	.	+	0	ID=annotation302713;Name=PenI;Parent=BBa_R0074
BBa_I14104	.	terminator	1473	1513	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04740
BBa_I14104	.	engineered_region	1473	1513	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I14104	.	stop_codon	1506	1506	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I14104	.	stem_loop	1480	1499	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I14104	.	polyA_site	1500	1513	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I14104	.	terminator	1385	1464	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04740
BBa_I14104	.	stem_loop	1396	1439	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I14104	.	engineered_region	1385	1464	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I14104
aaagaggggaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaaaataccacagat
ttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctc
agtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccacc
ataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttct
caaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatc
aatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtag
cttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagtcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaaga
ctacatttgtaagatttg