BBa_I14104
type: ComponentDefinition
persistent identity: BBa I14104
display id: BBa_I14104
version: 1
was generated by: iGEM to SBOL conversion
description: tetR + penI inverters in series
created: 2006-06-21T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: built from parts in the registry
owned by: james
owned by: myers
mutable description: tetR inverter followed by penI inverter -- made to test for possible bistable switches using pSB1A10
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 546
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: built from parts in the registry
star: false
creator: Kelly Chang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_Q04740
component: BBa_Q04401
sequence annotation: BBa_Q04401
sequence annotation: BBa_Q04740
sequence: https://synbiohub.org/public/igem/BBa_I14104_sequence/1

BBa_Q04740
type: Component
persistent identity: component2259987
display id: component2259987
version: 1
top level: BBa_I14104
definition: BBa_Q04740
access: public

BBa_Q04401
type: Component
persistent identity: component2259968
display id: component2259968
version: 1
top level: BBa_I14104
definition: tetR inver
access: public

BBa_Q04401
type: SequenceAnnotation
persistent identity: annotation2259968
display id: annotation2259968
version: 1
top level: BBa_I14104
location: https://synbiohub.org/public/igem/BBa_I14104/annotation2259968/range2259968/1
component: BBa_Q04401

https://synbiohub.org/public/igem/BBa_I14104/annotation2259968/range2259968/1
type: Range
persistent identity: range2259968
display id: range2259968
version: 1
top level: BBa_I14104
direction: direction/reverse
start: 1
end: 902
orientation: inline

BBa_Q04740
type: SequenceAnnotation
persistent identity: annotation2259987
display id: annotation2259987
version: 1
top level: BBa_I14104
location: https://synbiohub.org/public/igem/BBa_I14104/annotation2259987/range2259987/1
component: BBa_Q04740

https://synbiohub.org/public/igem/BBa_I14104/annotation2259987/range2259987/1
type: Range
persistent identity: range2259987
display id: range2259987
version: 1
top level: BBa_I14104
direction: direction/reverse
start: 911
end: 1598
orientation: inline

BBa_Q04740
type: ComponentDefinition
persistent identity: BBa Q04740
display id: BBa_Q04740
version: 1
was generated by: iGEM to SBOL conversion
description: Pen1 quad part inverter
created: 2004-07-04T11:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Crackdots
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Inverter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_R0074
component: BBa_C0074
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0074
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0074
sequence: https://synbiohub.org/public/igem/BBa_Q04740_sequence/1

BBa_B0012
type: Component
persistent identity: component953128
display id: component953128
version: 1
top level: BBa_Q04740
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component953118
display id: component953118
version: 1
top level: BBa_Q04740
definition: BBa_B0010
access: public

BBa_R0074
type: Component
persistent identity: component953148
display id: component953148
version: 1
top level: BBa_Q04740
definition: PenI
access: public

BBa_C0074
type: Component
persistent identity: component953112
display id: component953112
version: 1
top level: BBa_Q04740
definition: penI
access: public

BBa_B0034
type: Component
persistent identity: component953102
display id: component953102
version: 1
top level: BBa_Q04740
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation953102
display id: annotation953102
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953102/range953102/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04740/annotation953102/range953102/1
type: Range
persistent identity: range953102
display id: range953102
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0074
type: SequenceAnnotation
persistent identity: annotation953112
display id: annotation953112
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953112/range953112/1
component: BBa_C0074

https://synbiohub.org/public/igem/BBa_Q04740/annotation953112/range953112/1
type: Range
persistent identity: range953112
display id: range953112
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 19
end: 441
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation953118
display id: annotation953118
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953118/range953118/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04740/annotation953118/range953118/1
type: Range
persistent identity: range953118
display id: range953118
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 475
end: 554
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation953128
display id: annotation953128
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953128/range953128/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04740/annotation953128/range953128/1
type: Range
persistent identity: range953128
display id: range953128
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 563
end: 603
orientation: inline

BBa_R0074
type: SequenceAnnotation
persistent identity: annotation953148
display id: annotation953148
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953148/range953148/1
component: BBa_R0074

https://synbiohub.org/public/igem/BBa_Q04740/annotation953148/range953148/1
type: Range
persistent identity: range953148
display id: range953148
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 612
end: 688
orientation: inline

tetR inver
type: ComponentDefinition
persistent identity: BBa Q04401
display id: BBa_Q04401
version: 1
was generated by: iGEM to SBOL conversion
description: TetR-derived transcriptional inverter
created: 2006-05-10T11:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: Currently entered as a placeholder. This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS: AAAGAGGAGAAA to AAAGAGGGGAAA
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0064
component: BBa_B0015
component: BBa_C0040
component: BBa_R0040
sequence annotation: BBa_B0064
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_Q04401_sequence/1

BBa_B0064
type: Component
persistent identity: component2251459
display id: component2251459
version: 1
top level: tetR inver
definition: BBa_B0064
access: public

BBa_B0015
type: Component
persistent identity: component2251470
display id: component2251470
version: 1
top level: tetR inver
definition: BBa_B0015
access: public

BBa_C0040
type: Component
persistent identity: component2251463
display id: component2251463
version: 1
top level: tetR inver
definition: tetR
access: public

BBa_R0040
type: Component
persistent identity: component2251471
display id: component2251471
version: 1
top level: tetR inver
definition: p(tetR)
access: public

BBa_B0064
type: SequenceAnnotation
persistent identity: annotation2251459
display id: annotation2251459
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251459/range2251459/1
component: BBa_B0064

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251459/range2251459/1
type: Range
persistent identity: range2251459
display id: range2251459
version: 1
top level: tetR inver
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2251463
display id: annotation2251463
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251463/range2251463/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251463/range2251463/1
type: Range
persistent identity: range2251463
display id: range2251463
version: 1
top level: tetR inver
direction: direction/reverse
start: 19
end: 703
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251470
display id: annotation2251470
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251470/range2251470/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251470/range2251470/1
type: Range
persistent identity: range2251470
display id: range2251470
version: 1
top level: tetR inver
direction: direction/reverse
start: 712
end: 840
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2251471
display id: annotation2251471
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251471/range2251471/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251471/range2251471/1
type: Range
persistent identity: range2251471
display id: range2251471
version: 1
top level: tetR inver
direction: direction/forward
start: 849
end: 902
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_B0064
type: ComponentDefinition
persistent identity: BBa B0064
display id: BBa_B0064
version: 1
was generated by: iGEM to SBOL conversion
description: Tuned RBS for Q04401
created: 2008-02-23T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: This is a single bp change from B0034. It's strength is approximately 35% that of B0034.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Jason Kelly
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B0064_sequence/1

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (PenI regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: extends slightly past -50
star: true
creator: crackdots
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PenI
sequence annotation: -35
sequence annotation: dimer left half
sequence annotation: -10
sequence annotation: dimer right half
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
top level: PenI
direction: direction/forward
start: 1
end: 77
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
top level: PenI
direction: direction/forward
start: 23
end: 28
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
top level: PenI
direction: direction/forward
start: 31
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
top level: PenI
direction: direction/forward
start: 46
end: 51
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
top level: PenI
direction: direction/forward
start: 55
end: 76
orientation: inline

penI
type: ComponentDefinition
persistent identity: BBa C0074
display id: BBa_C0074
version: 1
was generated by: iGEM to SBOL conversion
description: penI repressor from Bacillus licheniformis (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Since penI is being ported from Bacillus Licheniformis, the sequence was changed within the coding region to reflect codon usage in E.Coli K12. Codons were mapped to the codon of the same usage rank in E.Coli.
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: penI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0074_sequence/1

penI
type: SequenceAnnotation
persistent identity: annotation302638
display id: annotation302638
version: 1
top level: penI
location: https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
type: Range
persistent identity: range302638
display id: range302638
version: 1
top level: penI
direction: direction/forward
start: 1
end: 387
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306563
display id: annotation306563
version: 1
top level: penI
location: https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
type: Range
persistent identity: range306563
display id: range306563
version: 1
top level: penI
direction: direction/forward
start: 385
end: 417
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214009
display id: annotation2214009
version: 1
top level: penI
location: https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
type: Range
persistent identity: range2214009
display id: range2214009
version: 1
top level: penI
direction: direction/forward
start: 424
end: 448
orientation: inline

https://synbiohub.org/public/igem/BBa_I14104_sequence/1
type: Sequence
persistent identity: BBa I14104 sequence
display id: BBa_I14104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04740_sequence/1
type: Sequence
persistent identity: BBa Q04740 sequence
display id: BBa_Q04740_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04401_sequence/1
type: Sequence
persistent identity: BBa Q04401 sequence
display id: BBa_Q04401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0064_sequence/1
type: Sequence
persistent identity: BBa B0064 sequence
display id: BBa_B0064_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0074_sequence/1
type: Sequence
persistent identity: BBa R0074 sequence
display id: BBa_R0074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0074_sequence/1
type: Sequence
persistent identity: BBa C0074 sequence
display id: BBa_C0074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z