LOCUS       BBa_I15000               778 bp    DNA     linear       15-May-2024
DEFINITION  Lux activated cI repressed LacZalpha
ACCESSION   BBa_I15000
VERSION     BBa_I15000.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..234
                     /label=BBa_R0065
     misc_feature    143..162
                     /label=Lux Box
     promoter        184..189
                     /label=-10
     misc_feature    194..210
                     /label=OR2 cI
     promoter        195..195
                     /label=lux p(R) start
     promoter        208..213
                     /label=-35
     misc_feature    218..234
                     /label=OR1 cI
     promoter        231..234
                     /label=-10
     promoter        138..234
                     /label=BBa_R0065
     RBS             243..257
                     /label=RBS-1\Strong
     RBS             250..254
                     /label=RBS
     misc_feature    243..257
                     /label=BBa_B0030
     RBS             243..257
                     /label=BBa_B0030
     CDS             264..611
                     /label=lacZ alpha
     misc_feature    344..344
                     /label=T
     misc_feature    350..350
                     /label=A
     misc_feature    371..371
                     /label=C
     misc_feature    341..341
                     /label=C
     misc_feature    374..374
                     /label=G
     promoter        289..298
                     /label=T3 promoter
     promoter        436..454
                     /label=T7 promoter
     misc_feature    462..611
                     /label=lacZ gene fragment
     misc_feature    264..278
                     /label=lacZ gene fragment
     misc_feature    264..616
                     /label=BBa_E0033
     stem_loop       661..704
                     /label=stem_loop
     misc_feature    650..729
                     /label=BBa_B0010
     terminator      650..729
                     /label=BBa_B0010
     stem_loop       745..764
                     /label=T7 TE
     misc_feature    771..771
                     /label=stop
     polyA_site      765..778
                     /label=polya
     misc_feature    738..778
                     /label=BBa_B0012
     terminator      738..778
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtaa gcacctgtag gatcgtacag gtttacgcaa gaaaatggtt
      181 tgttatagtc gaataacacc gtgcgtgttg actattttac ctctggcggt gatatactag
      241 agattaaaga ggagaaatac tagatgacca tgattacgcc aagcgcgcaa ttaaccctca
      301 ctaaagggaa caaaagctgg agctccaccg cggtggcggc agcactagag ctagtggatc
      361 ccccgggctg tagaaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
      421 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
      481 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      541 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      601 gcagcctgaa taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
      661 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      721 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//