BBa_I15001
type: ComponentDefinition
persistent identity: BBa I15001
display id: BBa_I15001
version: 1
was generated by: iGEM to SBOL conversion
description: LacI regulated cI/LuxI with constitutive LuxR
created: 2004-05-22T11:00:00Z
modified: 2015-08-31T04:07:38Z
owned by: james
owned by: myers
mutable description: Polycistronic lambda cI and LuxI proteins regulated by Lac Repressor (LacI). Downstream is consitutive LuxR expression cassette. System flanked by dual transcription terminators.
discontinued: true
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: jtabor
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_R1075
component: BBa_C0062
component: BBa_B0034
component: BBa_I1020
component: BBa_C0061
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I1020
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_R1075
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_I15001_sequence/1

BBa_B0012
type: Component
persistent identity: component974810
display id: component974810
version: 1
top level: BBa_I15001
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component974800
display id: component974800
version: 1
top level: BBa_I15001
definition: BBa_B0010
access: public

BBa_R1075
type: Component
persistent identity: component974904
display id: component974904
version: 1
top level: BBa_I15001
definition: const.
access: public

BBa_B0012
type: Component
persistent identity: component974893
display id: component974893
version: 1
top level: BBa_I15001
definition: BBa_B0012
access: public

BBa_C0062
type: Component
persistent identity: component974924
display id: component974924
version: 1
top level: BBa_I15001
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component974909
display id: component974909
version: 1
top level: BBa_I15001
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component974866
display id: component974866
version: 1
top level: BBa_I15001
definition: BBa_B0034
access: public

BBa_I1020
type: Component
persistent identity: component974847
display id: component974847
version: 1
top level: BBa_I15001
definition: BBa_I1020
access: public

BBa_B0010
type: Component
persistent identity: component974883
display id: component974883
version: 1
top level: BBa_I15001
definition: BBa_B0010
access: public

BBa_C0061
type: Component
persistent identity: component974876
display id: component974876
version: 1
top level: BBa_I15001
definition: luxI
access: public

BBa_B0012
type: Component
persistent identity: component974942
display id: component974942
version: 1
top level: BBa_I15001
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component974932
display id: component974932
version: 1
top level: BBa_I15001
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation974800
display id: annotation974800
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974800/range974800/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I15001/annotation974800/range974800/1
type: Range
persistent identity: range974800
display id: range974800
version: 1
top level: BBa_I15001
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation974810
display id: annotation974810
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974810/range974810/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I15001/annotation974810/range974810/1
type: Range
persistent identity: range974810
display id: range974810
version: 1
top level: BBa_I15001
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I1020
type: SequenceAnnotation
persistent identity: annotation974847
display id: annotation974847
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974847/range974847/1
component: BBa_I1020

https://synbiohub.org/public/igem/BBa_I15001/annotation974847/range974847/1
type: Range
persistent identity: range974847
display id: range974847
version: 1
top level: BBa_I15001
direction: direction/forward
start: 138
end: 972
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation974866
display id: annotation974866
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974866/range974866/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I15001/annotation974866/range974866/1
type: Range
persistent identity: range974866
display id: range974866
version: 1
top level: BBa_I15001
direction: direction/forward
start: 981
end: 992
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation974876
display id: annotation974876
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974876/range974876/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_I15001/annotation974876/range974876/1
type: Range
persistent identity: range974876
display id: range974876
version: 1
top level: BBa_I15001
direction: direction/forward
start: 999
end: 1616
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation974883
display id: annotation974883
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974883/range974883/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I15001/annotation974883/range974883/1
type: Range
persistent identity: range974883
display id: range974883
version: 1
top level: BBa_I15001
direction: direction/forward
start: 1650
end: 1729
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation974893
display id: annotation974893
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974893/range974893/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I15001/annotation974893/range974893/1
type: Range
persistent identity: range974893
display id: range974893
version: 1
top level: BBa_I15001
direction: direction/forward
start: 1738
end: 1778
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation974904
display id: annotation974904
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974904/range974904/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I15001/annotation974904/range974904/1
type: Range
persistent identity: range974904
display id: range974904
version: 1
top level: BBa_I15001
direction: direction/forward
start: 1787
end: 1835
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation974909
display id: annotation974909
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974909/range974909/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I15001/annotation974909/range974909/1
type: Range
persistent identity: range974909
display id: range974909
version: 1
top level: BBa_I15001
direction: direction/forward
start: 1844
end: 1855
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation974924
display id: annotation974924
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974924/range974924/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I15001/annotation974924/range974924/1
type: Range
persistent identity: range974924
display id: range974924
version: 1
top level: BBa_I15001
direction: direction/forward
start: 1862
end: 2617
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation974932
display id: annotation974932
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974932/range974932/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I15001/annotation974932/range974932/1
type: Range
persistent identity: range974932
display id: range974932
version: 1
top level: BBa_I15001
direction: direction/forward
start: 2651
end: 2730
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation974942
display id: annotation974942
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974942/range974942/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I15001/annotation974942/range974942/1
type: Range
persistent identity: range974942
display id: range974942
version: 1
top level: BBa_I15001
direction: direction/forward
start: 2739
end: 2779
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive Promoter II
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
top level: const.
direction: direction/forward
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
top level: const.
direction: direction/forward
start: 35
end: 40
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_I1020
type: ComponentDefinition
persistent identity: BBa I1020
display id: BBa_I1020
version: 1
was generated by: iGEM to SBOL conversion
description: Custom cI(2) Protein w/LacO-1 Promoter
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
mutable provenance: Modified from BioBricks <bb_part>BBa_R0011</bb_part>, and <bb_part>BBa_C0051</bb_part>.
owned by: james
owned by: myers
mutable description: Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: <bb_part>BBa_I1020</bb_part> Custom cI Protein with LacO-1 Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part> and <bb_part>BBa_R0011</bb_part>. It is designed such that <bb_part>BBa_I1023</bb_part> can be used to interfere with the <bb_part>BBa_1020</bb_part> transcript by anti-sense mRNA binding, in the IS10 method shown below. <bb_part>BBa_I1020</bb_part> and <bb_part>BBa_I1023</bb_part> can act indepently of related biobrick parts <bb_part>BBa_1010</bb_part>, <bb_part>BBa_1011</bb_part>, <bb_part>BBa_1012</bb_part>, <bb_part>BBa_I1030</bb_part>, <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1060</bb_part>, <bb_part>BBa_I1063</bb_part> and <bb_part>BBa_1013</bb_part>. Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1023. Incompatible with systems containing cI (wild type)
star: false
creator: Samantha Sutton, Neelaksh Varshney, Daniel Shen, Grace Kenney
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: LacO-1 region
sequence annotation: BBa_I1020
sequence annotation: -35
sequence annotation: LacO-1
sequence annotation: -10
sequence annotation: start
sequence annotation: stem_loop
sequence annotation: Complementary to Antisense mRNA I1023
sequence annotation: HH117a mutation specific to I1020 and I1023
sequence annotation: rbs
sequence annotation: cI lambda
sequence annotation: C added
sequence annotation: Homology to I1023
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_I1020_sequence/1

LacO-1 region
type: SequenceAnnotation
persistent identity: annotation1830
display id: annotation1830
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1830/range1830/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I1020/annotation1830/range1830/1
type: Range
persistent identity: range1830
display id: range1830
version: 1
top level: BBa_I1020
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_I1020
type: SequenceAnnotation
persistent identity: annotation7046
display id: annotation7046
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation7046/range7046/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I1020/annotation7046/range7046/1
type: Range
persistent identity: range7046
display id: range7046
version: 1
top level: BBa_I1020
direction: direction/forward
start: 1
end: 835
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1825
display id: annotation1825
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1825/range1825/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I1020/annotation1825/range1825/1
type: Range
persistent identity: range1825
display id: range1825
version: 1
top level: BBa_I1020
direction: direction/forward
start: 21
end: 26
orientation: inline

LacO-1
type: SequenceAnnotation
persistent identity: annotation1831
display id: annotation1831
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1831/range1831/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I1020/annotation1831/range1831/1
type: Range
persistent identity: range1831
display id: range1831
version: 1
top level: BBa_I1020
direction: direction/forward
start: 27
end: 43
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1826
display id: annotation1826
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1826/range1826/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I1020/annotation1826/range1826/1
type: Range
persistent identity: range1826
display id: range1826
version: 1
top level: BBa_I1020
direction: direction/forward
start: 44
end: 49
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1832
display id: annotation1832
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1832/range1832/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I1020/annotation1832/range1832/1
type: Range
persistent identity: range1832
display id: range1832
version: 1
top level: BBa_I1020
direction: direction/forward
start: 56
end: 56
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1820
display id: annotation1820
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1820/range1820/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I1020/annotation1820/range1820/1
type: Range
persistent identity: range1820
display id: range1820
version: 1
top level: BBa_I1020
direction: direction/forward
start: 56
end: 85
orientation: inline

Complementary to Antisense mRNA I1023
type: SequenceAnnotation
persistent identity: annotation1827
display id: annotation1827
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1827/range1827/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I1020/annotation1827/range1827/1
type: Range
persistent identity: range1827
display id: range1827
version: 1
top level: BBa_I1020
direction: direction/forward
start: 56
end: 90
orientation: inline

HH117a mutation specific to I1020 and I1023
type: SequenceAnnotation
persistent identity: annotation1833
display id: annotation1833
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1833/range1833/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I1020/annotation1833/range1833/1
type: Range
persistent identity: range1833
display id: range1833
version: 1
top level: BBa_I1020
direction: direction/forward
start: 58
end: 58
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1821
display id: annotation1821
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1821/range1821/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I1020/annotation1821/range1821/1
type: Range
persistent identity: range1821
display id: range1821
version: 1
top level: BBa_I1020
direction: direction/forward
start: 73
end: 78
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation1824
display id: annotation1824
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1824/range1824/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I1020/annotation1824/range1824/1
type: Range
persistent identity: range1824
display id: range1824
version: 1
top level: BBa_I1020
direction: direction/forward
start: 83
end: 835
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1823
display id: annotation1823
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1823/range1823/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I1020/annotation1823/range1823/1
type: Range
persistent identity: range1823
display id: range1823
version: 1
top level: BBa_I1020
direction: direction/forward
start: 83
end: 85
orientation: inline

C added
type: SequenceAnnotation
persistent identity: annotation1834
display id: annotation1834
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1834/range1834/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I1020/annotation1834/range1834/1
type: Range
persistent identity: range1834
display id: range1834
version: 1
top level: BBa_I1020
direction: direction/forward
start: 86
end: 88
orientation: inline

Homology to I1023
type: SequenceAnnotation
persistent identity: annotation1835
display id: annotation1835
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1835/range1835/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I1020/annotation1835/range1835/1
type: Range
persistent identity: range1835
display id: range1835
version: 1
top level: BBa_I1020
direction: direction/forward
start: 89
end: 90
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1828
display id: annotation1828
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1828/range1828/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_I1020/annotation1828/range1828/1
type: Range
persistent identity: range1828
display id: range1828
version: 1
top level: BBa_I1020
direction: direction/forward
start: 797
end: 835
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1829
display id: annotation1829
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1829/range1829/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I1020/annotation1829/range1829/1
type: Range
persistent identity: range1829
display id: range1829
version: 1
top level: BBa_I1020
direction: direction/forward
start: 830
end: 835
orientation: inline

https://synbiohub.org/public/igem/BBa_I15001_sequence/1
type: Sequence
persistent identity: BBa I15001 sequence
display id: BBa_I15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacgccaaaaatcaataatcagacaacaagatgtgcagtactaagaaaaagcctttgactcaggaacaactcgaagatgcccgccgtctcaaggccatctacgagaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1075_sequence/1
type: Sequence
persistent identity: BBa R1075 sequence
display id: BBa_R1075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I1020_sequence/1
type: Sequence
persistent identity: BBa I1020 sequence
display id: BBa_I1020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacgccaaaaatcaataatcagacaacaagatgtgcagtactaagaaaaagcctttgactcaggaacaactcgaagatgcccgccgtctcaaggccatctacgagaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z