LOCUS       BBa_I15002              2182 bp    DNA     linear       22-Nov-2024
DEFINITION  LacI regulated cI/LuxI/LacZalpha
ACCESSION   BBa_I15002
VERSION     BBa_I15002.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     stem_loop       193..222
                     /label=stem_loop
     RBS             210..215
                     /label=rbs
     misc_feature    220..222
                     /label=start
     CDS             220..972
                     /label=cI lambda
     promoter        158..163
                     /label=-35
     promoter        181..186
                     /label=-10
     misc_feature    193..227
                     /label=Complementary to Antisense mRNA I1023
     misc_feature    934..972
                     /label=SsrA
     misc_feature    967..972
                     /label=2
     misc_feature    138..157
                     /label=LacO-1 region
     misc_feature    164..180
                     /label=LacO-1
     misc_feature    193..193
                     /label=start
     misc_feature    195..195
                     /label=HH117a mutation specific to I1020 and I1023
     misc_feature    223..225
                     /label=C added
     misc_feature    226..227
                     /label=Homology to I1023
     misc_feature    138..972
                     /label=BBa_I1020
     misc_feature    138..972
                     /label=BBa_I1020
     misc_feature    985..988
                     /label=conserved
     RBS             981..992
                     /label=BBa_B0034
     misc_feature    1578..1609
                     /label=LVA
     CDS             999..1577
                     /label=luxI
     misc_feature    999..1616
                     /label=BBa_C0061
     misc_feature    1617..1641
                     /label=Help:Barcodes
     CDS             999..1616
                     /label=BBa_C0061
     misc_feature    1654..1657
                     /label=conserved
     RBS             1650..1661
                     /label=BBa_B0034
     CDS             1668..2015
                     /label=lacZ alpha
     misc_feature    1748..1748
                     /label=T
     misc_feature    1754..1754
                     /label=A
     misc_feature    1775..1775
                     /label=C
     misc_feature    1745..1745
                     /label=C
     misc_feature    1778..1778
                     /label=G
     promoter        1693..1702
                     /label=T3 promoter
     promoter        1840..1858
                     /label=T7 promoter
     misc_feature    1866..2015
                     /label=lacZ gene fragment
     misc_feature    1668..1682
                     /label=lacZ gene fragment
     misc_feature    1668..2020
                     /label=BBa_E0033
     stem_loop       2065..2108
                     /label=stem_loop
     misc_feature    2054..2133
                     /label=BBa_B0010
     terminator      2054..2133
                     /label=BBa_B0010
     stem_loop       2149..2168
                     /label=T7 TE
     misc_feature    2175..2175
                     /label=stop
     polyA_site      2169..2182
                     /label=polya
     misc_feature    2142..2182
                     /label=BBa_B0012
     terminator      2142..2182
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagata aatgtgagcg gataacattg acattgtgag cggataacaa
      181 gatactgagc acgccaaaaa tcaataatca gacaacaaga tgtgcagtac taagaaaaag
      241 cctttgactc aggaacaact cgaagatgcc cgccgtctca aggccatcta cgagaaaaag
      301 aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat ggggcagtca
      361 ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc cgcattgctt
      421 gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag agaaatctac
      481 gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga gtaccctgtt
      541 ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac caaaggtgat
      601 gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt
      661 gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga cggaatgtta
      721 attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc cagacttggg
      781 ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt tttacaacca
      841 ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt ggggaaagtt
      901 atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa ctacgcttta
      961 gtagcttaat aatactagag aaagaggaga aatactagat gactataatg ataaaaaaat
     1021 cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag
     1081 tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt
     1141 atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat
     1201 gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc
     1261 ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag
     1321 gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag
     1381 ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag
     1441 caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa
     1501 ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta
     1561 aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct taataactct
     1621 gatagtgcta gtgtagatct ctactagaga aagaggagaa atactagatg accatgatta
     1681 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg
     1741 cggcagcact agagctagtg gatcccccgg gctgtagaaa ttcgatatca agcttatcga
     1801 taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg
     1861 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1921 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1981 ccgatcgccc ttcccaacag ttgcgcagcc tgaataataa cgctgatagt gctagtgtag
     2041 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     2101 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     2161 ggtgggcctt tctgcgttta ta
//