BBa_I15002
type: ComponentDefinition
persistent identity: BBa I15002
display id: BBa_I15002
version: 1
was generated by: iGEM to SBOL conversion
description: LacI regulated cI/LuxI/LacZalpha
created: 2004-05-22T11:00:00Z
modified: 2015-08-31T04:07:38Z
owned by: james
owned by: myers
mutable description: Tester module for BBa_I15001. Flanked by dual terminators. LacI regulated polycistronic cI/LuxI/LacZalpha
discontinued: true
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: jtabor
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0033
component: BBa_B0034
component: BBa_I1020
component: BBa_C0061
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I1020
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_E0033
sequence: https://synbiohub.org/public/igem/BBa_I15002_sequence/1

BBa_B0012
type: Component
persistent identity: component962791
display id: component962791
version: 1
top level: BBa_I15002
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component962892
display id: component962892
version: 1
top level: BBa_I15002
definition: BBa_B0010
access: public

BBa_B0010
type: Component
persistent identity: component962781
display id: component962781
version: 1
top level: BBa_I15002
definition: BBa_B0010
access: public

BBa_E0033
type: Component
persistent identity: component962886
display id: component962886
version: 1
top level: BBa_I15002
definition: lacZ a
access: public

BBa_B0012
type: Component
persistent identity: component962902
display id: component962902
version: 1
top level: BBa_I15002
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component962847
display id: component962847
version: 1
top level: BBa_I15002
definition: BBa_B0034
access: public

BBa_I1020
type: Component
persistent identity: component962828
display id: component962828
version: 1
top level: BBa_I15002
definition: BBa_I1020
access: public

BBa_B0034
type: Component
persistent identity: component962864
display id: component962864
version: 1
top level: BBa_I15002
definition: BBa_B0034
access: public

BBa_C0061
type: Component
persistent identity: component962857
display id: component962857
version: 1
top level: BBa_I15002
definition: luxI
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation962781
display id: annotation962781
version: 1
top level: BBa_I15002
location: https://synbiohub.org/public/igem/BBa_I15002/annotation962781/range962781/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I15002/annotation962781/range962781/1
type: Range
persistent identity: range962781
display id: range962781
version: 1
top level: BBa_I15002
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation962791
display id: annotation962791
version: 1
top level: BBa_I15002
location: https://synbiohub.org/public/igem/BBa_I15002/annotation962791/range962791/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I15002/annotation962791/range962791/1
type: Range
persistent identity: range962791
display id: range962791
version: 1
top level: BBa_I15002
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I1020
type: SequenceAnnotation
persistent identity: annotation962828
display id: annotation962828
version: 1
top level: BBa_I15002
location: https://synbiohub.org/public/igem/BBa_I15002/annotation962828/range962828/1
component: BBa_I1020

https://synbiohub.org/public/igem/BBa_I15002/annotation962828/range962828/1
type: Range
persistent identity: range962828
display id: range962828
version: 1
top level: BBa_I15002
direction: direction/forward
start: 138
end: 972
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation962847
display id: annotation962847
version: 1
top level: BBa_I15002
location: https://synbiohub.org/public/igem/BBa_I15002/annotation962847/range962847/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I15002/annotation962847/range962847/1
type: Range
persistent identity: range962847
display id: range962847
version: 1
top level: BBa_I15002
direction: direction/forward
start: 981
end: 992
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation962857
display id: annotation962857
version: 1
top level: BBa_I15002
location: https://synbiohub.org/public/igem/BBa_I15002/annotation962857/range962857/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_I15002/annotation962857/range962857/1
type: Range
persistent identity: range962857
display id: range962857
version: 1
top level: BBa_I15002
direction: direction/forward
start: 999
end: 1616
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation962864
display id: annotation962864
version: 1
top level: BBa_I15002
location: https://synbiohub.org/public/igem/BBa_I15002/annotation962864/range962864/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I15002/annotation962864/range962864/1
type: Range
persistent identity: range962864
display id: range962864
version: 1
top level: BBa_I15002
direction: direction/forward
start: 1650
end: 1661
orientation: inline

BBa_E0033
type: SequenceAnnotation
persistent identity: annotation962886
display id: annotation962886
version: 1
top level: BBa_I15002
location: https://synbiohub.org/public/igem/BBa_I15002/annotation962886/range962886/1
component: BBa_E0033

https://synbiohub.org/public/igem/BBa_I15002/annotation962886/range962886/1
type: Range
persistent identity: range962886
display id: range962886
version: 1
top level: BBa_I15002
direction: direction/forward
start: 1668
end: 2020
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation962892
display id: annotation962892
version: 1
top level: BBa_I15002
location: https://synbiohub.org/public/igem/BBa_I15002/annotation962892/range962892/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I15002/annotation962892/range962892/1
type: Range
persistent identity: range962892
display id: range962892
version: 1
top level: BBa_I15002
direction: direction/forward
start: 2054
end: 2133
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation962902
display id: annotation962902
version: 1
top level: BBa_I15002
location: https://synbiohub.org/public/igem/BBa_I15002/annotation962902/range962902/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I15002/annotation962902/range962902/1
type: Range
persistent identity: range962902
display id: range962902
version: 1
top level: BBa_I15002
direction: direction/forward
start: 2142
end: 2182
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacZ a
type: ComponentDefinition
persistent identity: BBa E0033
display id: BBa_E0033
version: 1
was generated by: iGEM to SBOL conversion
description: LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
mutable description: to greatly reduce the number of bases, this is only a portion of the LacZ gene
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Restriction sites (modified) 76-79 (gcc to gca) 79-81 (gct to gca) 85-87 (gaa to gag) 106-108 (tgc to tgt) 109-111(agg to aga)
star: true
creator: Yong-Su Jin (Fighting Darwins)
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lacZ alpha
sequence annotation: lacZ gene fragment
sequence annotation: T3 promoter
sequence annotation: C
sequence annotation: T
sequence annotation: A
sequence annotation: G
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_E0033_sequence/1

lacZ alpha
type: SequenceAnnotation
persistent identity: annotation308320
display id: annotation308320
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1
type: Range
persistent identity: range308320
display id: range308320
version: 1
top level: lacZ a
direction: direction/forward
start: 1
end: 348
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938942
display id: annotation1938942
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1
type: Range
persistent identity: range1938942
display id: range1938942
version: 1
top level: lacZ a
direction: direction/forward
start: 1
end: 15
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1938939
display id: annotation1938939
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1
type: Range
persistent identity: range1938939
display id: range1938939
version: 1
top level: lacZ a
direction: direction/forward
start: 26
end: 35
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation308387
display id: annotation308387
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1
type: Range
persistent identity: range308387
display id: range308387
version: 1
top level: lacZ a
direction: direction/forward
start: 78
end: 78
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308375
display id: annotation308375
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1
type: Range
persistent identity: range308375
display id: range308375
version: 1
top level: lacZ a
direction: direction/forward
start: 81
end: 81
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation308381
display id: annotation308381
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1
type: Range
persistent identity: range308381
display id: range308381
version: 1
top level: lacZ a
direction: direction/forward
start: 87
end: 87
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation308383
display id: annotation308383
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1
type: Range
persistent identity: range308383
display id: range308383
version: 1
top level: lacZ a
direction: direction/forward
start: 108
end: 108
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308388
display id: annotation308388
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1
type: Range
persistent identity: range308388
display id: range308388
version: 1
top level: lacZ a
direction: direction/forward
start: 111
end: 111
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1938940
display id: annotation1938940
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1
type: Range
persistent identity: range1938940
display id: range1938940
version: 1
top level: lacZ a
direction: direction/reverse
start: 173
end: 191
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938941
display id: annotation1938941
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1
type: Range
persistent identity: range1938941
display id: range1938941
version: 1
top level: lacZ a
direction: direction/forward
start: 199
end: 348
orientation: inline

BBa_I1020
type: ComponentDefinition
persistent identity: BBa I1020
display id: BBa_I1020
version: 1
was generated by: iGEM to SBOL conversion
description: Custom cI(2) Protein w/LacO-1 Promoter
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
mutable provenance: Modified from BioBricks <bb_part>BBa_R0011</bb_part>, and <bb_part>BBa_C0051</bb_part>.
owned by: james
owned by: myers
mutable description: Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: <bb_part>BBa_I1020</bb_part> Custom cI Protein with LacO-1 Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part> and <bb_part>BBa_R0011</bb_part>. It is designed such that <bb_part>BBa_I1023</bb_part> can be used to interfere with the <bb_part>BBa_1020</bb_part> transcript by anti-sense mRNA binding, in the IS10 method shown below. <bb_part>BBa_I1020</bb_part> and <bb_part>BBa_I1023</bb_part> can act indepently of related biobrick parts <bb_part>BBa_1010</bb_part>, <bb_part>BBa_1011</bb_part>, <bb_part>BBa_1012</bb_part>, <bb_part>BBa_I1030</bb_part>, <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1060</bb_part>, <bb_part>BBa_I1063</bb_part> and <bb_part>BBa_1013</bb_part>. Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1023. Incompatible with systems containing cI (wild type)
star: false
creator: Samantha Sutton, Neelaksh Varshney, Daniel Shen, Grace Kenney
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: LacO-1 region
sequence annotation: BBa_I1020
sequence annotation: -35
sequence annotation: LacO-1
sequence annotation: -10
sequence annotation: start
sequence annotation: stem_loop
sequence annotation: Complementary to Antisense mRNA I1023
sequence annotation: HH117a mutation specific to I1020 and I1023
sequence annotation: rbs
sequence annotation: cI lambda
sequence annotation: C added
sequence annotation: Homology to I1023
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_I1020_sequence/1

LacO-1 region
type: SequenceAnnotation
persistent identity: annotation1830
display id: annotation1830
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1830/range1830/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I1020/annotation1830/range1830/1
type: Range
persistent identity: range1830
display id: range1830
version: 1
top level: BBa_I1020
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_I1020
type: SequenceAnnotation
persistent identity: annotation7046
display id: annotation7046
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation7046/range7046/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I1020/annotation7046/range7046/1
type: Range
persistent identity: range7046
display id: range7046
version: 1
top level: BBa_I1020
direction: direction/forward
start: 1
end: 835
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1825
display id: annotation1825
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1825/range1825/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I1020/annotation1825/range1825/1
type: Range
persistent identity: range1825
display id: range1825
version: 1
top level: BBa_I1020
direction: direction/forward
start: 21
end: 26
orientation: inline

LacO-1
type: SequenceAnnotation
persistent identity: annotation1831
display id: annotation1831
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1831/range1831/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I1020/annotation1831/range1831/1
type: Range
persistent identity: range1831
display id: range1831
version: 1
top level: BBa_I1020
direction: direction/forward
start: 27
end: 43
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1826
display id: annotation1826
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1826/range1826/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I1020/annotation1826/range1826/1
type: Range
persistent identity: range1826
display id: range1826
version: 1
top level: BBa_I1020
direction: direction/forward
start: 44
end: 49
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1832
display id: annotation1832
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1832/range1832/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I1020/annotation1832/range1832/1
type: Range
persistent identity: range1832
display id: range1832
version: 1
top level: BBa_I1020
direction: direction/forward
start: 56
end: 56
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1820
display id: annotation1820
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1820/range1820/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I1020/annotation1820/range1820/1
type: Range
persistent identity: range1820
display id: range1820
version: 1
top level: BBa_I1020
direction: direction/forward
start: 56
end: 85
orientation: inline

Complementary to Antisense mRNA I1023
type: SequenceAnnotation
persistent identity: annotation1827
display id: annotation1827
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1827/range1827/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I1020/annotation1827/range1827/1
type: Range
persistent identity: range1827
display id: range1827
version: 1
top level: BBa_I1020
direction: direction/forward
start: 56
end: 90
orientation: inline

HH117a mutation specific to I1020 and I1023
type: SequenceAnnotation
persistent identity: annotation1833
display id: annotation1833
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1833/range1833/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I1020/annotation1833/range1833/1
type: Range
persistent identity: range1833
display id: range1833
version: 1
top level: BBa_I1020
direction: direction/forward
start: 58
end: 58
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1821
display id: annotation1821
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1821/range1821/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I1020/annotation1821/range1821/1
type: Range
persistent identity: range1821
display id: range1821
version: 1
top level: BBa_I1020
direction: direction/forward
start: 73
end: 78
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation1824
display id: annotation1824
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1824/range1824/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I1020/annotation1824/range1824/1
type: Range
persistent identity: range1824
display id: range1824
version: 1
top level: BBa_I1020
direction: direction/forward
start: 83
end: 835
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1823
display id: annotation1823
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1823/range1823/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I1020/annotation1823/range1823/1
type: Range
persistent identity: range1823
display id: range1823
version: 1
top level: BBa_I1020
direction: direction/forward
start: 83
end: 85
orientation: inline

C added
type: SequenceAnnotation
persistent identity: annotation1834
display id: annotation1834
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1834/range1834/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I1020/annotation1834/range1834/1
type: Range
persistent identity: range1834
display id: range1834
version: 1
top level: BBa_I1020
direction: direction/forward
start: 86
end: 88
orientation: inline

Homology to I1023
type: SequenceAnnotation
persistent identity: annotation1835
display id: annotation1835
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1835/range1835/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I1020/annotation1835/range1835/1
type: Range
persistent identity: range1835
display id: range1835
version: 1
top level: BBa_I1020
direction: direction/forward
start: 89
end: 90
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1828
display id: annotation1828
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1828/range1828/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_I1020/annotation1828/range1828/1
type: Range
persistent identity: range1828
display id: range1828
version: 1
top level: BBa_I1020
direction: direction/forward
start: 797
end: 835
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1829
display id: annotation1829
version: 1
top level: BBa_I1020
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1829/range1829/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I1020/annotation1829/range1829/1
type: Range
persistent identity: range1829
display id: range1829
version: 1
top level: BBa_I1020
direction: direction/forward
start: 830
end: 835
orientation: inline

https://synbiohub.org/public/igem/BBa_I15002_sequence/1
type: Sequence
persistent identity: BBa I15002 sequence
display id: BBa_I15002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacgccaaaaatcaataatcagacaacaagatgtgcagtactaagaaaaagcctttgactcaggaacaactcgaagatgcccgccgtctcaaggccatctacgagaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0033_sequence/1
type: Sequence
persistent identity: BBa E0033 sequence
display id: BBa_E0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I1020_sequence/1
type: Sequence
persistent identity: BBa I1020 sequence
display id: BBa_I1020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacgccaaaaatcaataatcagacaacaagatgtgcagtactaagaaaaagcctttgactcaggaacaactcgaagatgcccgccgtctcaaggccatctacgagaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z