LOCUS       BBa_I15003               750 bp    DNA     linear       22-Nov-2024
DEFINITION  3OC6HSL+LuxR dependent LacZalpha
ACCESSION   BBa_I15003
VERSION     BBa_I15003.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    138..234
                     /label=BBa_R0065
     misc_feature    143..162
                     /label=Lux Box
     promoter        184..189
                     /label=-10
     misc_feature    194..210
                     /label=OR2 cI
     promoter        195..195
                     /label=lux p(R) start
     promoter        208..213
                     /label=-35
     misc_feature    218..234
                     /label=OR1 cI
     promoter        231..234
                     /label=-10
     promoter        138..234
                     /label=BBa_R0065
     misc_feature    247..250
                     /label=conserved
     RBS             243..254
                     /label=BBa_B0034
     CDS             261..608
                     /label=lacZ alpha
     misc_feature    341..341
                     /label=T
     misc_feature    347..347
                     /label=A
     misc_feature    368..368
                     /label=C
     misc_feature    338..338
                     /label=C
     misc_feature    371..371
                     /label=G
     promoter        286..295
                     /label=T3 promoter
     promoter        433..451
                     /label=T7 promoter
     misc_feature    459..608
                     /label=lacZ gene fragment
     misc_feature    261..275
                     /label=lacZ gene fragment
     misc_feature    261..613
                     /label=BBa_E0033
     stem_loop       633..676
                     /label=stem_loop
     misc_feature    622..701
                     /label=BBa_B0010
     terminator      622..701
                     /label=BBa_B0010
     stem_loop       717..736
                     /label=T7 TE
     misc_feature    743..743
                     /label=stop
     polyA_site      737..750
                     /label=polya
     misc_feature    710..750
                     /label=BBa_B0012
     terminator      710..750
                     /label=BBa_B0012
     terminator      622..750
                     /label=BBa_B0015
     misc_feature    243..750
                     /label=BBa_E0433
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtaa gcacctgtag gatcgtacag gtttacgcaa gaaaatggtt
      181 tgttatagtc gaataacacc gtgcgtgttg actattttac ctctggcggt gatatactag
      241 agaaagagga gaaatactag atgaccatga ttacgccaag cgcgcaatta accctcacta
      301 aagggaacaa aagctggagc tccaccgcgg tggcggcagc actagagcta gtggatcccc
      361 cgggctgtag aaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
      421 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      601 gcctgaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      661 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      721 caccttcggg tgggcctttc tgcgtttata
//