BBa_I15003
type: ComponentDefinition
persistent identity: BBa I15003
display id: BBa_I15003
version: 1
was generated by: iGEM to SBOL conversion
description: 3OC6HSL+LuxR dependent LacZalpha
created: 2004-05-25T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: formerly I15000
owned by: james
owned by: myers
mutable description: System flanked on both sides by dual transcription terminators. Bears a joint Lux promoter-lambda operator, strong RBS, with LacZ alpha peptide. Requires LacZ omega complementation. See notes on E0033. System dominantly repressed by lambda cI.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Jeff Tabor
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_E0433
component: BBa_R0065
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_R0065
sequence annotation: BBa_E0433
sequence: https://synbiohub.org/public/igem/BBa_I15003_sequence/1

BBa_E0433
type: Component
persistent identity: component2224909
display id: component2224909
version: 1
top level: BBa_I15003
definition: BBa_E0433
access: public

BBa_R0065
type: Component
persistent identity: component2224881
display id: component2224881
version: 1
top level: BBa_I15003
definition: cI+luxR
access: public

BBa_B0015
type: Component
persistent identity: component2224880
display id: component2224880
version: 1
top level: BBa_I15003
definition: BBa_B0015
access: public

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2224880
display id: annotation2224880
version: 1
top level: BBa_I15003
location: https://synbiohub.org/public/igem/BBa_I15003/annotation2224880/range2224880/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I15003/annotation2224880/range2224880/1
type: Range
persistent identity: range2224880
display id: range2224880
version: 1
top level: BBa_I15003
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation2224881
display id: annotation2224881
version: 1
top level: BBa_I15003
location: https://synbiohub.org/public/igem/BBa_I15003/annotation2224881/range2224881/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_I15003/annotation2224881/range2224881/1
type: Range
persistent identity: range2224881
display id: range2224881
version: 1
top level: BBa_I15003
direction: direction/forward
start: 138
end: 234
orientation: inline

BBa_E0433
type: SequenceAnnotation
persistent identity: annotation2224909
display id: annotation2224909
version: 1
top level: BBa_I15003
location: https://synbiohub.org/public/igem/BBa_I15003/annotation2224909/range2224909/1
component: BBa_E0433

https://synbiohub.org/public/igem/BBa_I15003/annotation2224909/range2224909/1
type: Range
persistent identity: range2224909
display id: range2224909
version: 1
top level: BBa_I15003
direction: direction/reverse
start: 243
end: 750
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_E0433
type: ComponentDefinition
persistent identity: BBa E0433
display id: BBa_E0433
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.E0033.B0015
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
mutable description: Reporter containing LacZ alpha fragment. Requires complementation with omega fragment. (See notes on E0033)
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_E0033
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_E0033
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_E0433_sequence/1

BBa_E0033
type: Component
persistent identity: component2220181
display id: component2220181
version: 1
top level: BBa_E0433
definition: lacZ a
access: public

BBa_B0034
type: Component
persistent identity: component2220170
display id: component2220170
version: 1
top level: BBa_E0433
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2220188
display id: component2220188
version: 1
top level: BBa_E0433
definition: BBa_B0015
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2220170
display id: annotation2220170
version: 1
top level: BBa_E0433
location: https://synbiohub.org/public/igem/BBa_E0433/annotation2220170/range2220170/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0433/annotation2220170/range2220170/1
type: Range
persistent identity: range2220170
display id: range2220170
version: 1
top level: BBa_E0433
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0033
type: SequenceAnnotation
persistent identity: annotation2220181
display id: annotation2220181
version: 1
top level: BBa_E0433
location: https://synbiohub.org/public/igem/BBa_E0433/annotation2220181/range2220181/1
component: BBa_E0033

https://synbiohub.org/public/igem/BBa_E0433/annotation2220181/range2220181/1
type: Range
persistent identity: range2220181
display id: range2220181
version: 1
top level: BBa_E0433
direction: direction/reverse
start: 19
end: 371
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2220188
display id: annotation2220188
version: 1
top level: BBa_E0433
location: https://synbiohub.org/public/igem/BBa_E0433/annotation2220188/range2220188/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_E0433/annotation2220188/range2220188/1
type: Range
persistent identity: range2220188
display id: range2220188
version: 1
top level: BBa_E0433
direction: direction/reverse
start: 380
end: 508
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lambda cI and luxR regulated -- hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0065
sequence annotation: Lux Box
sequence annotation: -10
sequence annotation: OR2 cI
sequence annotation: lux p(R) start
sequence annotation: -35
sequence annotation: OR1 cI
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
top level: cI+luxR
direction: direction/forward
start: 1
end: 97
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
top level: cI+luxR
direction: direction/forward
start: 6
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
top level: cI+luxR
direction: direction/forward
start: 47
end: 52
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
top level: cI+luxR
direction: direction/forward
start: 57
end: 73
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
top level: cI+luxR
direction: direction/forward
start: 58
end: 58
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
top level: cI+luxR
direction: direction/forward
start: 71
end: 76
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
top level: cI+luxR
direction: direction/forward
start: 81
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
top level: cI+luxR
direction: direction/forward
start: 94
end: 97
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacZ a
type: ComponentDefinition
persistent identity: BBa E0033
display id: BBa_E0033
version: 1
was generated by: iGEM to SBOL conversion
description: LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
mutable description: to greatly reduce the number of bases, this is only a portion of the LacZ gene
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Restriction sites (modified) 76-79 (gcc to gca) 79-81 (gct to gca) 85-87 (gaa to gag) 106-108 (tgc to tgt) 109-111(agg to aga)
star: true
creator: Yong-Su Jin (Fighting Darwins)
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lacZ alpha
sequence annotation: lacZ gene fragment
sequence annotation: T3 promoter
sequence annotation: C
sequence annotation: T
sequence annotation: A
sequence annotation: G
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_E0033_sequence/1

lacZ alpha
type: SequenceAnnotation
persistent identity: annotation308320
display id: annotation308320
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1
type: Range
persistent identity: range308320
display id: range308320
version: 1
top level: lacZ a
direction: direction/forward
start: 1
end: 348
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938942
display id: annotation1938942
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1
type: Range
persistent identity: range1938942
display id: range1938942
version: 1
top level: lacZ a
direction: direction/forward
start: 1
end: 15
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1938939
display id: annotation1938939
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1
type: Range
persistent identity: range1938939
display id: range1938939
version: 1
top level: lacZ a
direction: direction/forward
start: 26
end: 35
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation308387
display id: annotation308387
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1
type: Range
persistent identity: range308387
display id: range308387
version: 1
top level: lacZ a
direction: direction/forward
start: 78
end: 78
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308375
display id: annotation308375
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1
type: Range
persistent identity: range308375
display id: range308375
version: 1
top level: lacZ a
direction: direction/forward
start: 81
end: 81
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation308381
display id: annotation308381
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1
type: Range
persistent identity: range308381
display id: range308381
version: 1
top level: lacZ a
direction: direction/forward
start: 87
end: 87
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation308383
display id: annotation308383
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1
type: Range
persistent identity: range308383
display id: range308383
version: 1
top level: lacZ a
direction: direction/forward
start: 108
end: 108
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308388
display id: annotation308388
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1
type: Range
persistent identity: range308388
display id: range308388
version: 1
top level: lacZ a
direction: direction/forward
start: 111
end: 111
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1938940
display id: annotation1938940
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1
type: Range
persistent identity: range1938940
display id: range1938940
version: 1
top level: lacZ a
direction: direction/reverse
start: 173
end: 191
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938941
display id: annotation1938941
version: 1
top level: lacZ a
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1
type: Range
persistent identity: range1938941
display id: range1938941
version: 1
top level: lacZ a
direction: direction/forward
start: 199
end: 348
orientation: inline

https://synbiohub.org/public/igem/BBa_I15003_sequence/1
type: Sequence
persistent identity: BBa I15003 sequence
display id: BBa_I15003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0433_sequence/1
type: Sequence
persistent identity: BBa E0433 sequence
display id: BBa_E0433_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0065_sequence/1
type: Sequence
persistent identity: BBa R0065 sequence
display id: BBa_R0065_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0033_sequence/1
type: Sequence
persistent identity: BBa E0033 sequence
display id: BBa_E0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z