LOCUS BBa_I15004 2805 bp DNA linear 22-Nov-2024 DEFINITION LuxR generator + LacI regulated lambda cI (LVA)+LuxI (LVA) ACCESSION BBa_I15004 VERSION BBa_I15004.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 140..156 /label=lac O1 promoter 157..162 /label=-35 misc_feature 163..179 /label=lac O1 promoter 180..185 /label=-10 misc_feature 138..191 /label=BBa_R0011 promoter 138..191 /label=BBa_R0011 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 CDS 222..929 /label=cI lambda misc_feature 930..962 /label=LVA misc_feature 969..993 /label=Help:Barcodes CDS 219..993 /label=BBa_C0051 misc_feature 1006..1009 /label=conserved RBS 1002..1013 /label=BBa_B0034 misc_feature 1599..1630 /label=LVA CDS 1020..1598 /label=luxI misc_feature 1020..1637 /label=BBa_C0061 misc_feature 1638..1662 /label=Help:Barcodes CDS 1020..1637 /label=BBa_C0061 stem_loop 1682..1725 /label=stem_loop misc_feature 1671..1750 /label=BBa_B0010 terminator 1671..1750 /label=BBa_B0010 stem_loop 1766..1785 /label=T7 TE misc_feature 1792..1792 /label=stop polyA_site 1786..1799 /label=polya misc_feature 1759..1799 /label=BBa_B0012 terminator 1759..1799 /label=BBa_B0012 misc_feature 1002..1799 /label=BBa_F1610 misc_feature 1808..1826 /label=TetR 1 misc_feature 1808..1861 /label=BBa_R0040 promoter 1827..1832 /label=-35 misc_feature 1833..1851 /label=TetR 2 promoter 1850..1855 /label=-10 promoter 1808..1861 /label=BBa_R0040 misc_feature 1874..1877 /label=conserved RBS 1870..1881 /label=BBa_B0034 misc_feature 1888..1889 /label=prefix misc_feature 2061..2061 /label=T misc_feature 2379..2379 /label=A CDS 1888..2637 /label=luxR misc_feature 1888..2643 /label=BBa_C0062 misc_feature 2644..2668 /label=Help:Barcodes CDS 1888..2643 /label=BBa_C0062 stem_loop 2688..2731 /label=stem_loop misc_feature 2677..2756 /label=BBa_B0010 terminator 2677..2756 /label=BBa_B0010 stem_loop 2772..2791 /label=T7 TE misc_feature 2798..2798 /label=stop polyA_site 2792..2805 /label=polya misc_feature 2765..2805 /label=BBa_B0012 terminator 2765..2805 /label=BBa_B0012 misc_feature 1870..2805 /label=BBa_I0462 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag 181 atactgagca catactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat 241 taacacaaga gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa 301 atgaacttgg cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg 361 ttggtgcttt atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa 421 aaattctcaa agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga 481 tgtatgaagc ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt 541 ctcatgttca ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg 601 agagatgggt aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag 661 gtaattccat gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc 721 tcgttgaccc tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg 781 atgagtttac cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa 841 acccacagta cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg 901 ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag 961 cttaataacg ctgatagtgc tagtgtagat cgctactaga gaaagaggag aaatactaga 1021 tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta 1081 ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa 1141 ataaccttga atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg 1201 atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga 1261 aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg 1321 aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa 1381 ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat 1441 atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc 1501 atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta 1561 tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg 1621 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca 1681 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 1741 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 1801 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca 1861 ctactagaga aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga 1921 ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat 1981 atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg 2041 gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca atattatgat 2101 gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca 2161 attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa 2221 gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat 2281 ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt 2341 ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa 2401 ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta 2461 gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag 2521 cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc 2581 caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa 2641 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 2701 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 2761 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //