LOCUS       BBa_I15005              2178 bp    DNA     linear       09-May-2024
DEFINITION  LacI repressed lambda cI(LVA) + LuxI(LVA) + LacZalpha
ACCESSION   BBa_I15005
VERSION     BBa_I15005.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    140..156
                     /label=lac O1
     promoter        157..162
                     /label=-35
     misc_feature    163..179
                     /label=lac O1
     promoter        180..185
                     /label=-10
     misc_feature    138..191
                     /label=BBa_R0011
     promoter        138..191
                     /label=BBa_R0011
     misc_feature    205..208
                     /label=conserved
     RBS             201..212
                     /label=BBa_B0034
     CDS             222..929
                     /label=cI lambda
     misc_feature    930..962
                     /label=LVA
     misc_feature    969..993
                     /label=Help:Barcodes
     CDS             219..993
                     /label=BBa_C0051
     misc_feature    1006..1009
                     /label=conserved
     RBS             1002..1013
                     /label=BBa_B0034
     misc_feature    1599..1630
                     /label=LVA
     CDS             1020..1598
                     /label=luxI
     misc_feature    1020..1637
                     /label=BBa_C0061
     misc_feature    1638..1662
                     /label=Help:Barcodes
     CDS             1020..1637
                     /label=BBa_C0061
     misc_feature    1675..1678
                     /label=conserved
     RBS             1671..1682
                     /label=BBa_B0034
     CDS             1689..2036
                     /label=lacZ alpha
     misc_feature    1769..1769
                     /label=T
     misc_feature    1775..1775
                     /label=A
     misc_feature    1796..1796
                     /label=C
     misc_feature    1766..1766
                     /label=C
     misc_feature    1799..1799
                     /label=G
     promoter        1714..1723
                     /label=T3 promoter
     promoter        1861..1879
                     /label=T7 promoter
     misc_feature    1887..2036
                     /label=lacZ gene fragment
     misc_feature    1689..1703
                     /label=lacZ gene fragment
     misc_feature    1689..2041
                     /label=BBa_E0033
     stem_loop       2061..2104
                     /label=stem_loop
     misc_feature    2050..2129
                     /label=BBa_B0010
     terminator      2050..2129
                     /label=BBa_B0010
     stem_loop       2145..2164
                     /label=T7 TE
     misc_feature    2171..2171
                     /label=stop
     polyA_site      2165..2178
                     /label=polya
     misc_feature    2138..2178
                     /label=BBa_B0012
     terminator      2138..2178
                     /label=BBa_B0012
     terminator      2050..2178
                     /label=BBa_B0015
     misc_feature    1671..2178
                     /label=BBa_E0433
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag
      181 atactgagca catactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat
      241 taacacaaga gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa
      301 atgaacttgg cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg
      361 ttggtgcttt atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa
      421 aaattctcaa agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga
      481 tgtatgaagc ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt
      541 ctcatgttca ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg
      601 agagatgggt aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag
      661 gtaattccat gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc
      721 tcgttgaccc tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg
      781 atgagtttac cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa
      841 acccacagta cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg
      901 ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag
      961 cttaataacg ctgatagtgc tagtgtagat cgctactaga gaaagaggag aaatactaga
     1021 tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta
     1081 ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa
     1141 ataaccttga atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg
     1201 atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga
     1261 aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg
     1321 aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa
     1381 ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat
     1441 atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc
     1501 atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta
     1561 tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg
     1621 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga
     1681 aatactagat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     1741 gctggagctc caccgcggtg gcggcagcac tagagctagt ggatcccccg ggctgtagaa
     1801 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
     1861 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     1921 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1981 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaataata
     2041 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2101 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2161 ggcctttctg cgtttata
//