LOCUS BBa_I15022 2858 bp DNA linear 24-Nov-2024 DEFINITION pOmpR dependent cI (LVA+) and LuxI (LVA+) generator ACCESSION BBa_I15022 VERSION BBa_I15022.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 misc_feature 150..167 /label=C1 OmpR misc_feature 171..188 /label=C2 OmpR misc_feature 191..208 /label=C3 OmpR promoter 212..217 /label=-35 promoter 235..240 /label=-10 promoter 138..245 /label=BBa_R0082 misc_feature 258..261 /label=conserved RBS 254..265 /label=BBa_B0034 CDS 275..982 /label=cI lambda misc_feature 983..1015 /label=LVA misc_feature 1022..1046 /label=Help:Barcodes CDS 272..1021 /label=BBa_C0051 misc_feature 1059..1062 /label=conserved RBS 1055..1066 /label=BBa_B0034 misc_feature 1652..1683 /label=LVA CDS 1073..1651 /label=luxI misc_feature 1073..1690 /label=BBa_C0061 misc_feature 1691..1715 /label=Help:Barcodes CDS 1073..1690 /label=BBa_C0061 stem_loop 1735..1778 /label=stem_loop misc_feature 1724..1803 /label=BBa_B0010 terminator 1724..1803 /label=BBa_B0010 stem_loop 1819..1838 /label=T7 TE misc_feature 1845..1845 /label=stop polyA_site 1839..1852 /label=polya misc_feature 1812..1852 /label=BBa_B0012 terminator 1812..1852 /label=BBa_B0012 misc_feature 1861..1879 /label=TetR 1 misc_feature 1861..1914 /label=BBa_R0040 promoter 1880..1885 /label=-35 misc_feature 1886..1904 /label=TetR 2 promoter 1903..1908 /label=-10 promoter 1861..1914 /label=BBa_R0040 misc_feature 1927..1930 /label=conserved RBS 1923..1934 /label=BBa_B0034 misc_feature 1941..1942 /label=prefix misc_feature 2114..2114 /label=T misc_feature 2432..2432 /label=A CDS 1941..2690 /label=luxR misc_feature 1941..2696 /label=BBa_C0062 misc_feature 2697..2721 /label=Help:Barcodes CDS 1941..2696 /label=BBa_C0062 stem_loop 2741..2784 /label=stem_loop misc_feature 2730..2809 /label=BBa_B0010 terminator 2730..2809 /label=BBa_B0010 stem_loop 2825..2844 /label=T7 TE misc_feature 2851..2851 /label=stop polyA_site 2845..2858 /label=polya misc_feature 2818..2858 /label=BBa_B0012 terminator 2818..2858 /label=BBa_B0012 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagtcc cttgcattta cattttgaaa catctatagc gataaatgaa 181 acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt tggggagaat 241 ggacttacta gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca 301 agagcagctt gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact 361 tggcttatcc caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc 421 tttatttaat ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct 481 caaagttagc gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga 541 agcggttagt atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt 601 tcaggcaggg atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg 661 ggtaagcaca accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc 721 catgaccgca ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga 781 ccctgagcag gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt 841 taccttcaag aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca 901 gtacccaatg atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca 961 gtggcctgaa gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata 1021 acgctgatag tgctagtgta gatcgctact agagaaagag gagaaatact agatgactat 1081 aatgataaaa aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag 1141 tcttcgttat caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct 1201 tgaatcagat gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga 1261 aaatgtaagt ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt 1321 ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag 1381 tcgttttgct gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat 1441 gaaactattt gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac 1501 agtaacatca acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat 1561 tggagacaaa gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat 1621 taatgaacag tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact acgctttagt 1681 agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa 1741 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 1801 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 1861 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 1921 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 1981 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 2041 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 2101 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 2161 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 2221 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 2281 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 2341 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 2401 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 2461 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 2521 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 2581 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 2641 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 2701 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 2761 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 2821 cactggctca ccttcgggtg ggcctttctg cgtttata //