##gff-version 3
##sequence-region BBa_I15025 1 3898
BBa_I15025	.	engineered_region	1	2858	.	+	0	ID=BBa_I15022;Name=BBa_I15022
BBa_I15025	.	terminator	2730	2809	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I15022
BBa_I15025	.	engineered_region	2730	2809	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15025	.	stem_loop	2741	2784	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15025	.	promoter	1861	1914	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I15022
BBa_I15025	.	engineered_region	1861	1914	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I15025	.	non_covalent_binding_site	1861	1879	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I15025	.	promoter	1903	1908	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I15025	.	promoter	1880	1885	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I15025	.	non_covalent_binding_site	1886	1904	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I15025	.	CDS	272	1021	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I15022
BBa_I15025	.	tag	983	1015	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I15025	.	CDS	275	982	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I15025	.	engineered_tag	1022	1046	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I15025	.	terminator	1724	1803	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I15022
BBa_I15025	.	engineered_region	1724	1803	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15025	.	stem_loop	1735	1778	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15025	.	ribosome_entry_site	254	265	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I15022
BBa_I15025	.	conserved_region	258	261	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15025	.	promoter	138	245	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_I15022
BBa_I15025	.	non_covalent_binding_site	191	208	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_I15025	.	promoter	235	240	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_I15025	.	promoter	212	217	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_I15025	.	non_covalent_binding_site	150	167	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_I15025	.	non_covalent_binding_site	171	188	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_I15025	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I15022
BBa_I15025	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15025	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15025	.	ribosome_entry_site	1923	1934	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I15022
BBa_I15025	.	conserved_region	1927	1930	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15025	.	terminator	2818	2858	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I15022
BBa_I15025	.	polyA_site	2845	2858	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15025	.	engineered_region	2818	2858	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15025	.	stem_loop	2825	2844	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15025	.	stop_codon	2851	2851	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15025	.	CDS	1941	2696	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I15022
BBa_I15025	.	engineered_region	1941	2696	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I15025	.	engineered_region	1941	1942	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I15025	.	CDS	1941	2690	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I15025	.	point_mutation	2114	2114	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I15025	.	point_mutation	2432	2432	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I15025	.	engineered_tag	2697	2721	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I15025	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I15022
BBa_I15025	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15025	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15025	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15025	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15025	.	CDS	1073	1690	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_I15022
BBa_I15025	.	engineered_region	1073	1690	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I15025	.	engineered_tag	1691	1715	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I15025	.	CDS	1073	1651	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I15025	.	tag	1652	1683	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I15025	.	terminator	1812	1852	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I15022
BBa_I15025	.	polyA_site	1839	1852	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15025	.	engineered_region	1812	1852	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15025	.	stem_loop	1819	1838	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15025	.	stop_codon	1845	1845	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15025	.	ribosome_entry_site	1055	1066	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I15022
BBa_I15025	.	conserved_region	1059	1062	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15025	.	engineered_region	2867	3898	.	+	0	ID=BBa_S01656;Name=BBa_S01656
BBa_I15025	.	engineered_region	2930	3898	.	+	0	ID=BBa_I0460;Name=BBa_I0460;Parent=BBa_S01656
BBa_I15025	.	CDS	2948	3736	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_I15025	.	engineered_region	2948	3736	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_I15025	.	tag	3698	3730	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_I15025	.	start_codon	2948	2950	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_I15025	.	stop_codon	3731	3736	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_I15025	.	engineered_tag	3737	3761	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_I15025	.	CDS	2948	3697	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_I15025	.	terminator	3770	3849	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_I15025	.	engineered_region	3770	3849	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15025	.	stem_loop	3781	3824	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15025	.	terminator	3858	3898	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_I15025	.	polyA_site	3885	3898	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15025	.	engineered_region	3858	3898	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15025	.	stem_loop	3865	3884	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15025	.	stop_codon	3891	3891	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15025	.	ribosome_entry_site	2930	2941	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_I15025	.	conserved_region	2934	2937	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15025	.	promoter	2867	2920	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_S01656
BBa_I15025	.	operator	2869	2885	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I15025	.	operator	2892	2908	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I15025	.	promoter	2909	2914	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I15025	.	engineered_region	2867	2920	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I15025	.	promoter	2886	2891	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
>BBa_I15025
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgcatttacattttga
aacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggag
aatggacttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggac
gcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatgg
ggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaa
aattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatg
cagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaa
cctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttga
aggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcag
gctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcg
gtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagt
tatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctga
tagtgctagtgtagatcgctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttgg
caattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagt
tgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaat
gtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaac
agagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactc
tgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaaca
gtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatg
tattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaa
cgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagt
gatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaat
aattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgt
gaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaa
aatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcacc
aattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggt
cttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaag
acaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcg
aaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaa
ggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaa
tgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaa
aaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacata
ctagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcatt
cgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaagg
acctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaaggg
caggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttc
tttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtaca
gcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatc
attgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctat
taattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagt
gccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccg
attgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaa
actacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata