LOCUS       BBa_I15030              1909 bp    DNA     linear       22-Nov-2024
DEFINITION  3OC6HSL amplifier
ACCESSION   BBa_I15030
VERSION     BBa_I15030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     misc_feature    703..734
                     /label=LVA
     CDS             124..702
                     /label=luxI
     misc_feature    124..741
                     /label=BBa_C0061
     misc_feature    742..766
                     /label=Help:Barcodes
     CDS             124..741
                     /label=BBa_C0061
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    775..854
                     /label=BBa_B0010
     terminator      775..854
                     /label=BBa_B0010
     stem_loop       870..889
                     /label=T7 TE
     misc_feature    896..896
                     /label=stop
     polyA_site      890..903
                     /label=polya
     misc_feature    863..903
                     /label=BBa_B0012
     terminator      863..903
                     /label=BBa_B0012
     misc_feature    912..930
                     /label=TetR 1
     misc_feature    912..965
                     /label=BBa_R0040
     promoter        931..936
                     /label=-35
     misc_feature    937..955
                     /label=TetR 2
     promoter        954..959
                     /label=-10
     promoter        912..965
                     /label=BBa_R0040
     misc_feature    978..981
                     /label=conserved
     RBS             974..985
                     /label=BBa_B0034
     misc_feature    992..993
                     /label=prefix
     misc_feature    1165..1165
                     /label=T
     misc_feature    1483..1483
                     /label=A
     CDS             992..1741
                     /label=luxR
     misc_feature    992..1747
                     /label=BBa_C0062
     misc_feature    1748..1772
                     /label=Help:Barcodes
     CDS             992..1747
                     /label=BBa_C0062
     stem_loop       1792..1835
                     /label=stem_loop
     misc_feature    1781..1860
                     /label=BBa_B0010
     terminator      1781..1860
                     /label=BBa_B0010
     stem_loop       1876..1895
                     /label=T7 TE
     misc_feature    1902..1902
                     /label=stop
     polyA_site      1896..1909
                     /label=polya
     misc_feature    1869..1909
                     /label=BBa_B0012
     terminator      1869..1909
                     /label=BBa_B0012
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatgacta taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa
      181 ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta
      241 gaaaataacc ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt
      301 gatgatactg aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg
      361 ctgaaaagtg tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata
      421 gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt
      481 gaaattacaa tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca
      541 gaatatgtaa cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct
      601 tgtcatcgta ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg
      661 tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac
      721 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagccaggc
      781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg
      961 agcactacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata
     1021 cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc
     1081 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc
     1141 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta
     1201 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc
     1261 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat
     1321 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa
     1381 caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt
     1441 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg
     1501 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg
     1561 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag
     1621 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg
     1681 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa
     1741 ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga
     1801 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1861 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//