LOCUS BBa_I15031 2900 bp DNA linear 20-Nov-2024 DEFINITION 3OC6HSL amplifier + reporter device ACCESSION BBa_I15031 VERSION BBa_I15031.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 CDS 124..846 /label=BBa_E0030 stem_loop 866..909 /label=stem_loop misc_feature 855..934 /label=BBa_B0010 terminator 855..934 /label=BBa_B0010 stem_loop 950..969 /label=T7 TE misc_feature 976..976 /label=stop polyA_site 970..983 /label=polya misc_feature 943..983 /label=BBa_B0012 terminator 943..983 /label=BBa_B0012 misc_feature 992..1088 /label=BBa_R0065 misc_feature 997..1016 /label=Lux Box promoter 1038..1043 /label=-10 misc_feature 1048..1064 /label=OR2 cI promoter 1049..1049 /label=lux p(R) start promoter 1062..1067 /label=-35 misc_feature 1072..1088 /label=OR1 cI promoter 1085..1088 /label=-10 promoter 992..1088 /label=BBa_R0065 misc_feature 1101..1104 /label=conserved RBS 1097..1108 /label=BBa_B0034 misc_feature 1694..1725 /label=LVA CDS 1115..1693 /label=luxI misc_feature 1115..1732 /label=BBa_C0061 misc_feature 1733..1757 /label=Help:Barcodes CDS 1115..1732 /label=BBa_C0061 stem_loop 1777..1820 /label=stem_loop misc_feature 1766..1845 /label=BBa_B0010 terminator 1766..1845 /label=BBa_B0010 stem_loop 1861..1880 /label=T7 TE misc_feature 1887..1887 /label=stop polyA_site 1881..1894 /label=polya misc_feature 1854..1894 /label=BBa_B0012 terminator 1854..1894 /label=BBa_B0012 misc_feature 1903..1921 /label=TetR 1 misc_feature 1903..1956 /label=BBa_R0040 promoter 1922..1927 /label=-35 misc_feature 1928..1946 /label=TetR 2 promoter 1945..1950 /label=-10 promoter 1903..1956 /label=BBa_R0040 misc_feature 1969..1972 /label=conserved RBS 1965..1976 /label=BBa_B0034 misc_feature 1983..1984 /label=prefix misc_feature 2156..2156 /label=T misc_feature 2474..2474 /label=A CDS 1983..2732 /label=luxR misc_feature 1983..2738 /label=BBa_C0062 misc_feature 2739..2763 /label=Help:Barcodes CDS 1983..2738 /label=BBa_C0062 stem_loop 2783..2826 /label=stem_loop misc_feature 2772..2851 /label=BBa_B0010 terminator 2772..2851 /label=BBa_B0010 stem_loop 2867..2886 /label=T7 TE misc_feature 2893..2893 /label=stop polyA_site 2887..2900 /label=polya misc_feature 2860..2900 /label=BBa_B0012 terminator 2860..2900 /label=BBa_B0012 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 181 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 241 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 301 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg 361 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 421 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 481 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 541 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 601 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 661 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 721 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 781 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 841 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 901 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 961 gggtgggcct ttctgcgttt atatactaga gtaagcacct gtaggatcgt acaggtttac 1021 gcaagaaaat ggtttgttat agtcgaataa caccgtgcgt gttgactatt ttacctctgg 1081 cggtgatata ctagagaaag aggagaaata ctagatgact ataatgataa aaaaatcgga 1141 ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt atcaagtgtt 1201 taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag atgagtatga 1261 taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa gtggatgctg 1321 gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg aattgcttgg 1381 tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg ctgtaggtaa 1441 aaatagctca aagataaata actctgctag tgaaattaca atgaaactat ttgaagctat 1501 atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat caacagcaat 1561 agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca aagaaattca 1621 tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac agtttaaaaa 1681 agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata 1741 gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1801 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1861 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag 1921 attgacatcc ctatcagtga tagagatact gagcactact agagaaagag gagaaatact 1981 agatgaaaaa cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta 2041 gaagcaataa tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat 2101 attatttact cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag 2161 ataattaccc taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc 2221 ctatagtaga ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca 2281 atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca 2341 ctgggtttag tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac 2401 attcagaaaa agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat 2461 taattgttcc ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa 2521 acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct 2581 cttgggatat ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca 2641 atgcgcaaat gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa 2701 caggagcaat tgattgccca tactttaaaa attaataaca ctgatagtgc tagtgtagat 2761 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 2821 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 2881 tgggcctttc tgcgtttata //