LOCUS BBa_I15032 2858 bp DNA linear 20-Nov-2024 DEFINITION 3OC6HSL amplifier + reporter device ACCESSION BBa_I15032 VERSION BBa_I15032.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..804 /label=BBa_E0030 stem_loop 824..867 /label=stem_loop misc_feature 813..892 /label=BBa_B0010 terminator 813..892 /label=BBa_B0010 stem_loop 908..927 /label=T7 TE misc_feature 934..934 /label=stop polyA_site 928..941 /label=polya misc_feature 901..941 /label=BBa_B0012 terminator 901..941 /label=BBa_B0012 misc_feature 950..1046 /label=BBa_R0065 misc_feature 955..974 /label=Lux Box promoter 996..1001 /label=-10 misc_feature 1006..1022 /label=OR2 cI promoter 1007..1007 /label=lux p(R) start promoter 1020..1025 /label=-35 misc_feature 1030..1046 /label=OR1 cI promoter 1043..1046 /label=-10 promoter 950..1046 /label=BBa_R0065 misc_feature 1059..1062 /label=conserved RBS 1055..1066 /label=BBa_B0034 misc_feature 1652..1683 /label=LVA CDS 1073..1651 /label=luxI misc_feature 1073..1690 /label=BBa_C0061 misc_feature 1691..1715 /label=Help:Barcodes CDS 1073..1690 /label=BBa_C0061 stem_loop 1735..1778 /label=stem_loop misc_feature 1724..1803 /label=BBa_B0010 terminator 1724..1803 /label=BBa_B0010 stem_loop 1819..1838 /label=T7 TE misc_feature 1845..1845 /label=stop polyA_site 1839..1852 /label=polya misc_feature 1812..1852 /label=BBa_B0012 terminator 1812..1852 /label=BBa_B0012 misc_feature 1861..1879 /label=TetR 1 misc_feature 1861..1914 /label=BBa_R0040 promoter 1880..1885 /label=-35 misc_feature 1886..1904 /label=TetR 2 promoter 1903..1908 /label=-10 promoter 1861..1914 /label=BBa_R0040 misc_feature 1927..1930 /label=conserved RBS 1923..1934 /label=BBa_B0034 misc_feature 1941..1942 /label=prefix misc_feature 2114..2114 /label=T misc_feature 2432..2432 /label=A CDS 1941..2690 /label=luxR misc_feature 1941..2696 /label=BBa_C0062 misc_feature 2697..2721 /label=Help:Barcodes CDS 1941..2696 /label=BBa_C0062 stem_loop 2741..2784 /label=stem_loop misc_feature 2730..2809 /label=BBa_B0010 terminator 2730..2809 /label=BBa_B0010 stem_loop 2825..2844 /label=T7 TE misc_feature 2851..2851 /label=stop polyA_site 2845..2858 /label=polya misc_feature 2818..2858 /label=BBa_B0012 terminator 2818..2858 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca 841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt aagcacctgt 961 aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgaataaca ccgtgcgtgt 1021 tgactatttt acctctggcg gtgatatact agagaaagag gagaaatact agatgactat 1081 aatgataaaa aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag 1141 tcttcgttat caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct 1201 tgaatcagat gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga 1261 aaatgtaagt ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt 1321 ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag 1381 tcgttttgct gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat 1441 gaaactattt gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac 1501 agtaacatca acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat 1561 tggagacaaa gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat 1621 taatgaacag tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact acgctttagt 1681 agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa 1741 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 1801 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 1861 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 1921 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 1981 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 2041 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 2101 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 2161 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 2221 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 2281 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 2341 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 2401 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 2461 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 2521 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 2581 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 2641 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 2701 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 2761 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 2821 cactggctca ccttcgggtg ggcctttctg cgtttata //