LOCUS       BBa_I2000                931 bp    DNA     linear       25-Nov-2024
DEFINITION  Dimeric Zif268 fingers 2 and 3 regulated GFP generator
ACCESSION   BBa_I2000
VERSION     BBa_I2000.1  
FEATURES             Location/Qualifiers
     promoter        10..15
                     /label=-35
     promoter        33..38
                     /label=-10
     misc_feature    20..33
                     /label=Zif23
     misc_feature    1..9
                     /label=consensus
     misc_feature    45..45
                     /label=(+1)
     promoter        1..45
                     /label=BBa_R2000
     RBS             54..68
                     /label=RBS-1\Strong
     RBS             61..65
                     /label=RBS
     misc_feature    54..68
                     /label=BBa_B0030
     RBS             54..68
                     /label=BBa_B0030
     CDS             75..794
                     /label=GFP protein
     CDS             75..794
                     /label=BBa_E0040
     stem_loop       814..857
                     /label=stem_loop
     misc_feature    803..882
                     /label=BBa_B0010
     terminator      803..882
                     /label=BBa_B0010
     stem_loop       898..917
                     /label=T7 TE
     misc_feature    924..924
                     /label=stop
     polyA_site      918..931
                     /label=polya
     misc_feature    891..931
                     /label=BBa_B0012
     terminator      891..931
                     /label=BBa_B0012
ORIGIN
        1 agtttattct tgacatggtc ccacgcgcgt gggatactac gtcagtacta gagattaaag
       61 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      121 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      181 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      241 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
      301 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      361 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      421 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      481 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      541 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
      601 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      661 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
      721 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
      781 tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      841 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      901 tcaccttcgg gtgggccttt ctgcgtttat a
//