BBa_I20100
type: ComponentDefinition
persistent identity: BBa I20100
display id: BBa_I20100
version: 1
was generated by: iGEM to SBOL conversion
description: RBS Scaffold with GFP reporter
created: 2007-03-06T12:00:00Z
modified: 2015-08-31T04:07:39Z
mutable provenance: This is a composite part formed from subparts available in the registry.
owned by: james
owned by: myers
mutable description: This construct is a scaffold device for measuring RBS strength via GFP fluorescence. The device contains a ccdB expression cassette (BBa_P1010) in place of an RBS. Digestion with AvrII and SapI allows for the insertion of any RBS sequence via Heather Keller's alternative RBS assembly method, which allows for blunt cloning of an RBS upstream of a coding sequence.
discontinued: true
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Due to the presence of ccdB (BBa_P1010) this device can only be grown in DB3.1 or other permissive strains.
star: false
creator: Heather Keller
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_B0101
component: BBa_E0041
component: BBa_P1010
component: BBa_B0012
component: BBa_S00159
component: BBa_B0010
sequence annotation: BBa_S00159
sequence annotation: BBa_P1010
sequence annotation: BBa_E0041
sequence annotation: BBa_B0101
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I20100_sequence/1

BBa_B0101
type: Component
persistent identity: component1920609
display id: component1920609
version: 1
top level: BBa_I20100
definition: HP3 3
access: public

BBa_E0041
type: Component
persistent identity: component1920607
display id: component1920607
version: 1
top level: BBa_I20100
definition: BBa_E0041
access: public

BBa_P1010
type: Component
persistent identity: component1920603
display id: component1920603
version: 1
top level: BBa_I20100
definition: ccdB
access: public

BBa_B0012
type: Component
persistent identity: component1920612
display id: component1920612
version: 1
top level: BBa_I20100
definition: BBa_B0012
access: public

BBa_S00159
type: Component
persistent identity: component1920597
display id: component1920597
version: 1
top level: BBa_I20100
definition: BBa_S00159
access: public

BBa_B0010
type: Component
persistent identity: component1920610
display id: component1920610
version: 1
top level: BBa_I20100
definition: BBa_B0010
access: public

BBa_S00159
type: SequenceAnnotation
persistent identity: annotation1920597
display id: annotation1920597
version: 1
top level: BBa_I20100
location: https://synbiohub.org/public/igem/BBa_I20100/annotation1920597/range1920597/1
component: BBa_S00159

https://synbiohub.org/public/igem/BBa_I20100/annotation1920597/range1920597/1
type: Range
persistent identity: range1920597
display id: range1920597
version: 1
top level: BBa_I20100
direction: direction/reverse
start: 1
end: 176
orientation: inline

BBa_P1010
type: SequenceAnnotation
persistent identity: annotation1920603
display id: annotation1920603
version: 1
top level: BBa_I20100
location: https://synbiohub.org/public/igem/BBa_I20100/annotation1920603/range1920603/1
component: BBa_P1010

https://synbiohub.org/public/igem/BBa_I20100/annotation1920603/range1920603/1
type: Range
persistent identity: range1920603
display id: range1920603
version: 1
top level: BBa_I20100
direction: direction/reverse
start: 185
end: 859
orientation: inline

BBa_E0041
type: SequenceAnnotation
persistent identity: annotation1920607
display id: annotation1920607
version: 1
top level: BBa_I20100
location: https://synbiohub.org/public/igem/BBa_I20100/annotation1920607/range1920607/1
component: BBa_E0041

https://synbiohub.org/public/igem/BBa_I20100/annotation1920607/range1920607/1
type: Range
persistent identity: range1920607
display id: range1920607
version: 1
top level: BBa_I20100
direction: direction/reverse
start: 868
end: 1595
orientation: inline

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920609
display id: annotation1920609
version: 1
top level: BBa_I20100
location: https://synbiohub.org/public/igem/BBa_I20100/annotation1920609/range1920609/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_I20100/annotation1920609/range1920609/1
type: Range
persistent identity: range1920609
display id: range1920609
version: 1
top level: BBa_I20100
direction: direction/reverse
start: 1604
end: 1661
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920610
display id: annotation1920610
version: 1
top level: BBa_I20100
location: https://synbiohub.org/public/igem/BBa_I20100/annotation1920610/range1920610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I20100/annotation1920610/range1920610/1
type: Range
persistent identity: range1920610
display id: range1920610
version: 1
top level: BBa_I20100
direction: direction/forward
start: 1670
end: 1749
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920612
display id: annotation1920612
version: 1
top level: BBa_I20100
location: https://synbiohub.org/public/igem/BBa_I20100/annotation1920612/range1920612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I20100/annotation1920612/range1920612/1
type: Range
persistent identity: range1920612
display id: range1920612
version: 1
top level: BBa_I20100
direction: direction/forward
start: 1758
end: 1798
orientation: inline

HP3 3
type: ComponentDefinition
persistent identity: BBa B0101
display id: BBa_B0101
version: 1
was generated by: iGEM to SBOL conversion
description: HP3 3
created: 2007-02-28T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: The part was made by primer annealing.
owned by: james
owned by: myers
mutable description: 3' stabilizing mRNA designed by Christina Smolke and Jay Keasling for stabilizing gfp.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: A single base pair change (A to T at position 25) from the original design was made in order to remove a PstI site occurring in the loop of the hairpin. This should have no effect on the secondary structure.
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0101_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1919685
display id: annotation1919685
version: 1
top level: HP3 3
location: https://synbiohub.org/public/igem/BBa_B0101/annotation1919685/range1919685/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0101/annotation1919685/range1919685/1
type: Range
persistent identity: range1919685
display id: range1919685
version: 1
top level: HP3 3
direction: direction/forward
start: 6
end: 58
orientation: inline

BBa_S00159
type: ComponentDefinition
persistent identity: BBa S00159
display id: BBa_S00159
version: 1
was generated by: iGEM to SBOL conversion
description: R0011+B0100+B0048
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:16Z
mutable provenance: This part was generated by PCR amplification. The upstream biobricks cut sites (EcoRI and XbaI) as well as the R0011 promoter were incorporated into the forward promoter recognizing the ompA region in the MG1655 genome. The AvrII cut site, as well as the downstream biobricks cut sites (SpeI and PstI) were incorporated into the reverse promoter.
owned by: james
owned by: myers
mutable description: This part is an intermediate assembly product that contains the R0011 promoter (lambda cI promoter with lacI binding sites), the OmpaA 5' UTR containing a double hairpin for transcript stability, and an AvrII restriction site to facilitate later downstream cloning of an RBS via Heather Keller's RBS characterization scheme. These parts are cloned bluntly, without generating biobricks mixed sites in between.
discontinued: false
dominant: true
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part was generated by PCR assembly in order to minimize the number of digestions and ligations necessary to generate a larger construct. Especially given the short sequence of the three individual parts (and the difficulting in purifying small parts) it was much simpler to amplify the product in this way rather than generate the 3 parts individually and clone them together.
star: false
creator: Heather Keller
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_B0100
sequence annotation: hp1
sequence annotation: ss1
sequence annotation: hp2
sequence annotation: ss2
sequence annotation: AvrII site
sequence annotation: BBa_B0048
sequence: https://synbiohub.org/public/igem/BBa_S00159_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1920195
display id: annotation1920195
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920195/range1920195/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_S00159/annotation1920195/range1920195/1
type: Range
persistent identity: range1920195
display id: range1920195
version: 1
top level: BBa_S00159
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1920196
display id: annotation1920196
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920196/range1920196/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_S00159/annotation1920196/range1920196/1
type: Range
persistent identity: range1920196
display id: range1920196
version: 1
top level: BBa_S00159
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1920197
display id: annotation1920197
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920197/range1920197/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_S00159/annotation1920197/range1920197/1
type: Range
persistent identity: range1920197
display id: range1920197
version: 1
top level: BBa_S00159
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1920198
display id: annotation1920198
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920198/range1920198/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_S00159/annotation1920198/range1920198/1
type: Range
persistent identity: range1920198
display id: range1920198
version: 1
top level: BBa_S00159
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1920199
display id: annotation1920199
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920199/range1920199/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_S00159/annotation1920199/range1920199/1
type: Range
persistent identity: range1920199
display id: range1920199
version: 1
top level: BBa_S00159
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0100
type: SequenceAnnotation
persistent identity: annotation1920204
display id: annotation1920204
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920204/range1920204/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_S00159/annotation1920204/range1920204/1
type: Range
persistent identity: range1920204
display id: range1920204
version: 1
top level: BBa_S00159
direction: direction/reverse
start: 56
end: 170
orientation: inline

hp1
type: SequenceAnnotation
persistent identity: annotation1920200
display id: annotation1920200
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920200/range1920200/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_S00159/annotation1920200/range1920200/1
type: Range
persistent identity: range1920200
display id: range1920200
version: 1
top level: BBa_S00159
direction: direction/forward
start: 56
end: 118
orientation: inline

ss1
type: SequenceAnnotation
persistent identity: annotation1920201
display id: annotation1920201
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920201/range1920201/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_S00159/annotation1920201/range1920201/1
type: Range
persistent identity: range1920201
display id: range1920201
version: 1
top level: BBa_S00159
direction: direction/forward
start: 119
end: 129
orientation: inline

hp2
type: SequenceAnnotation
persistent identity: annotation1920202
display id: annotation1920202
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920202/range1920202/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_S00159/annotation1920202/range1920202/1
type: Range
persistent identity: range1920202
display id: range1920202
version: 1
top level: BBa_S00159
direction: direction/forward
start: 130
end: 158
orientation: inline

ss2
type: SequenceAnnotation
persistent identity: annotation1920203
display id: annotation1920203
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920203/range1920203/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_S00159/annotation1920203/range1920203/1
type: Range
persistent identity: range1920203
display id: range1920203
version: 1
top level: BBa_S00159
direction: direction/forward
start: 159
end: 170
orientation: inline

AvrII site
type: SequenceAnnotation
persistent identity: annotation1920205
display id: annotation1920205
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920205/range1920205/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_S00159/annotation1920205/range1920205/1
type: Range
persistent identity: range1920205
display id: range1920205
version: 1
top level: BBa_S00159
direction: direction/forward
start: 171
end: 176
orientation: inline

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation1920206
display id: annotation1920206
version: 1
top level: BBa_S00159
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920206/range1920206/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_S00159/annotation1920206/range1920206/1
type: Range
persistent identity: range1920206
display id: range1920206
version: 1
top level: BBa_S00159
direction: direction/reverse
start: 171
end: 176
orientation: inline

BBa_E0041
type: ComponentDefinition
persistent identity: BBa E0041
display id: BBa_E0041
version: 1
was generated by: iGEM to SBOL conversion
description: SapI + GFP
created: 2007-03-05T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: The part was generated by PCR amplification of part E0040 with the SapI site incorported into the forward promoter.
owned by: james
owned by: myers
mutable description: GFP (mut3) part number E0040 with a unpstream SapI site. Cleave with SapI results in an overhang of 3'-TAC - 5' from the left end of the GFP coding region, making the coding sequence compatible with Heather Keller's alternative assembly method for blunt cloning of RBS sequences upstream of a coding sequence.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Heather Keller
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: SapI recognition
sequence annotation: BBa_B0102
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_E0041_sequence/1

SapI recognition
type: SequenceAnnotation
persistent identity: annotation1920210
display id: annotation1920210
version: 1
top level: BBa_E0041
location: https://synbiohub.org/public/igem/BBa_E0041/annotation1920210/range1920210/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E0041/annotation1920210/range1920210/1
type: Range
persistent identity: range1920210
display id: range1920210
version: 1
top level: BBa_E0041
direction: direction/forward
start: 1
end: 7
orientation: inline

BBa_B0102
type: SequenceAnnotation
persistent identity: annotation1920211
display id: annotation1920211
version: 1
top level: BBa_E0041
location: https://synbiohub.org/public/igem/BBa_E0041/annotation1920211/range1920211/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0041/annotation1920211/range1920211/1
type: Range
persistent identity: range1920211
display id: range1920211
version: 1
top level: BBa_E0041
direction: direction/reverse
start: 1
end: 8
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1920212
display id: annotation1920212
version: 1
top level: BBa_E0041
location: https://synbiohub.org/public/igem/BBa_E0041/annotation1920212/range1920212/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_E0041/annotation1920212/range1920212/1
type: Range
persistent identity: range1920212
display id: range1920212
version: 1
top level: BBa_E0041
direction: direction/reverse
start: 9
end: 728
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1010
display id: BBa_P1010
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB cell death gene
created: 2004-07-27T11:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
part status: Deleted
mutable description: The ccd operon having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. This part is used to aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up. P1010 is only provided as plasmid or in DB3.1 which is ccdB resistant.
discontinued: true
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Leon Chan
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ccdB (rev)
sequence annotation: ccdA- (rev)
sequence annotation: T to C mutation
sequence annotation: -10 (rev)
sequence annotation: -35 (rev)
sequence: https://synbiohub.org/public/igem/BBa_P1010_sequence/1

ccdB (rev)
type: SequenceAnnotation
persistent identity: annotation1747332
display id: annotation1747332
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747332/range1747332/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_P1010/annotation1747332/range1747332/1
type: Range
persistent identity: range1747332
display id: range1747332
version: 1
top level: ccdB
direction: direction/reverse
start: 29
end: 334
orientation: inline

ccdA- (rev)
type: SequenceAnnotation
persistent identity: annotation1747333
display id: annotation1747333
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747333/range1747333/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_P1010/annotation1747333/range1747333/1
type: Range
persistent identity: range1747333
display id: range1747333
version: 1
top level: ccdB
direction: direction/reverse
start: 336
end: 556
orientation: inline

T to C mutation
type: SequenceAnnotation
persistent identity: annotation1747334
display id: annotation1747334
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747334/range1747334/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_P1010/annotation1747334/range1747334/1
type: Range
persistent identity: range1747334
display id: range1747334
version: 1
top level: ccdB
direction: direction/forward
start: 467
end: 467
orientation: inline

-10 (rev)
type: SequenceAnnotation
persistent identity: annotation1747335
display id: annotation1747335
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747335/range1747335/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_P1010/annotation1747335/range1747335/1
type: Range
persistent identity: range1747335
display id: range1747335
version: 1
top level: ccdB
direction: direction/reverse
start: 595
end: 600
orientation: inline

-35 (rev)
type: SequenceAnnotation
persistent identity: annotation1747336
display id: annotation1747336
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747336/range1747336/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_P1010/annotation1747336/range1747336/1
type: Range
persistent identity: range1747336
display id: range1747336
version: 1
top level: ccdB
direction: direction/reverse
start: 618
end: 623
orientation: inline

https://synbiohub.org/public/igem/BBa_I20100_sequence/1
type: Sequence
persistent identity: BBa I20100 sequence
display id: BBa_I20100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctaggtactagagactggctgtgtataagggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgttactagaggctcttctatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0101_sequence/1
type: Sequence
persistent identity: BBa B0101 sequence
display id: BBa_B0101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S00159_sequence/1
type: Sequence
persistent identity: BBa S00159 sequence
display id: BBa_S00159_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0041_sequence/1
type: Sequence
persistent identity: BBa E0041 sequence
display id: BBa_E0041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcttctatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1010_sequence/1
type: Sequence
persistent identity: BBa P1010 sequence
display id: BBa_P1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actggctgtgtataagggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z