LOCUS       BBa_I2020               1002 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive Promoter I Test (R0074.E0422)
ACCESSION   BBa_I2020
VERSION     BBa_I2020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..77
                     /label=PenI
     promoter        23..28
                     /label=-35
     promoter        46..51
                     /label=-10
     misc_feature    31..53
                     /label=dimer left half
     misc_feature    55..76
                     /label=dimer right half
     promoter        1..77
                     /label=BBa_R0074
     misc_feature    90..93
                     /label=conserved
     RBS             86..97
                     /label=BBa_B0034
     CDS             104..865
                     /label=CFP (LVA)
     misc_feature    822..859
                     /label=SsrA
     misc_feature    860..865
                     /label=2
     misc_feature    172..172
                     /label=A
     misc_feature    104..865
                     /label=BBa_E0022
     misc_feature    104..865
                     /label=BBa_E0022
     stem_loop       885..928
                     /label=stem_loop
     misc_feature    874..953
                     /label=BBa_B0010
     terminator      874..953
                     /label=BBa_B0010
     stem_loop       969..988
                     /label=T7 TE
     misc_feature    995..995
                     /label=stop
     polyA_site      989..1002
                     /label=polya
     misc_feature    962..1002
                     /label=BBa_B0012
     terminator      962..1002
                     /label=BBa_B0012
ORIGIN
        1 tcatttccaa ccgaaaaaag gcttgcattt aaatcttaca tatgtaatac tttcaaagac
       61 tacatttgta agatttgtac tagagaaaga ggagaaatac tagatggtga gcaagggcga
      121 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg tgaacggcca
      181 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      241 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      301 ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      361 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      421 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      481 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      541 catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
      601 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      661 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      721 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      781 cgccgccggg atcactctcg gcatggacga gctgtacaag aggcctgctg caaacgacga
      841 aaactacgct ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc
      901 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      961 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//