LOCUS BBa_I2022 3576 bp DNA linear 25-Nov-2024 DEFINITION cI857 Test (I0500.B0034.E0022.C0081.B0015.R0051.E0032) ACCESSION BBa_I2022 VERSION BBa_I2022.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1998 /label=CFP (LVA) misc_feature 1955..1992 /label=SsrA misc_feature 1993..1998 /label=2 misc_feature 1305..1305 /label=A misc_feature 1237..1998 /label=BBa_E0022 misc_feature 1237..1998 /label=BBa_E0022 stem_loop 2018..2061 /label=stem_loop misc_feature 2007..2086 /label=BBa_B0010 terminator 2007..2086 /label=BBa_B0010 stem_loop 2102..2121 /label=T7 TE misc_feature 2128..2128 /label=stop polyA_site 2122..2135 /label=polya misc_feature 2095..2135 /label=BBa_B0012 terminator 2095..2135 /label=BBa_B0012 misc_feature 1219..2135 /label=BBa_E0422 misc_feature 2148..2151 /label=conserved RBS 2144..2155 /label=BBa_B0034 CDS 2162..2509 /label=lacZ alpha misc_feature 2242..2242 /label=T misc_feature 2248..2248 /label=A misc_feature 2269..2269 /label=C misc_feature 2239..2239 /label=C misc_feature 2272..2272 /label=G promoter 2187..2196 /label=T3 promoter promoter 2334..2352 /label=T7 promoter misc_feature 2360..2509 /label=lacZ gene fragment misc_feature 2162..2176 /label=lacZ gene fragment misc_feature 2162..2514 /label=BBa_E0033 stem_loop 2534..2577 /label=stem_loop misc_feature 2523..2602 /label=BBa_B0010 terminator 2523..2602 /label=BBa_B0010 stem_loop 2618..2637 /label=T7 TE misc_feature 2644..2644 /label=stop polyA_site 2638..2651 /label=polya misc_feature 2611..2651 /label=BBa_B0012 terminator 2611..2651 /label=BBa_B0012 terminator 2523..2651 /label=BBa_B0015 misc_feature 2144..2651 /label=BBa_I2012 misc_feature 2664..2667 /label=conserved RBS 2660..2671 /label=BBa_B0034 CDS 2678..3439 /label=YFP (LVA) misc_feature 3434..3439 /label=2 misc_feature 3395..3433 /label=SsrA misc_feature 2919..2919 /label=A (Q->L) misc_feature 2678..3439 /label=BBa_E0032 misc_feature 2678..3439 /label=BBa_E0032 stem_loop 3459..3502 /label=stem_loop misc_feature 3448..3527 /label=BBa_B0010 terminator 3448..3527 /label=BBa_B0010 stem_loop 3543..3562 /label=T7 TE misc_feature 3569..3569 /label=stop polyA_site 3563..3576 /label=polya misc_feature 3536..3576 /label=BBa_B0012 terminator 3536..3576 /label=BBa_B0012 misc_feature 2660..3576 /label=BBa_E0432 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac 1981 gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2041 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2101 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaaagagg agaaatacta 2161 gatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag 2221 ctccaccgcg gtggcggcag cactagagct agtggatccc ccgggctgta gaaattcgat 2281 atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt cgccctatag 2341 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 2401 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 2461 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaata ataatactag 2521 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 2581 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 2641 ctgcgtttat atactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct 2701 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 2761 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 2821 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg 2881 cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct tcaagtccgc 2941 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 3001 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 3061 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 3121 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 3181 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 3241 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct 3301 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 3361 cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg acgaaaacta 3421 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 3481 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3541 ctggctcacc ttcgggtggg cctttctgcg tttata //