LOCUS       BBa_I20222              1113 bp    DNA     linear       25-Nov-2024
DEFINITION  4B RBS from T7 charactization device
ACCESSION   BBa_I20222
VERSION     BBa_I20222.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     stem_loop       56..118
                     /label=hp1
     misc_feature    119..129
                     /label=ss1
     stem_loop       130..158
                     /label=hp2
     misc_feature    159..170
                     /label=ss2
     misc_feature    56..170
                     /label=BBa_B0100
     misc_feature    171..176
                     /label=AvrII/XbaI mixed site
     misc_feature    171..176
                     /label=BBa_B0104
     misc_feature    1..176
                     /label=BBa_S00164
     RBS             177..196
                     /label=BBa_B2022
     CDS             197..907
                     /label=mCherry
     misc_feature    548..548
                     /label=C->T (removing PstI site)
     misc_feature    197..910
                     /label=BBa_J06504
     stem_loop       924..976
                     /label=stem_loop
     misc_feature    919..976
                     /label=BBa_B0101
     stem_loop       996..1039
                     /label=stem_loop
     misc_feature    985..1064
                     /label=BBa_B0010
     terminator      985..1064
                     /label=BBa_B0010
     stem_loop       1080..1099
                     /label=T7 TE
     misc_feature    1106..1106
                     /label=stop
     polyA_site      1100..1113
                     /label=polya
     misc_feature    1073..1113
                     /label=BBa_B0012
     terminator      1073..1113
                     /label=BBa_B0012
     misc_feature    197..1113
                     /label=BBa_S00162
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacagccag
       61 gggtgctcgg cataagccga agatatcggt agagttaata ttgagcagat cccccggtga
      121 aggatttaac cgtgttatct cgttggagat attcatggcg tattttggat cctagaaacc
      181 ctcaggaggt aaaccaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
      241 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      301 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      361 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      421 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      481 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      541 ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      601 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      661 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
      721 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      781 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      841 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      901 caagtaataa tactagagga tcgcctgatc ccggtgcacc cgggcagctg catagtctgg
      961 gtgcaccggg atcaggtact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1021 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1081 gctcaccttc gggtgggcct ttctgcgttt ata
//