LOCUS BBa_I2023 4087 bp DNA linear 22-Nov-2024 DEFINITION Full Heat Trigger Test (R1075.Q04811.E0432) ACCESSION BBa_I2023 VERSION BBa_I2023.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1998 /label=CFP (LVA) misc_feature 1955..1992 /label=SsrA misc_feature 1993..1998 /label=2 misc_feature 1305..1305 /label=A misc_feature 1237..1998 /label=BBa_E0022 misc_feature 1237..1998 /label=BBa_E0022 stem_loop 2018..2061 /label=stem_loop misc_feature 2007..2086 /label=BBa_B0010 terminator 2007..2086 /label=BBa_B0010 stem_loop 2102..2121 /label=T7 TE misc_feature 2128..2128 /label=stop polyA_site 2122..2135 /label=polya misc_feature 2095..2135 /label=BBa_B0012 terminator 2095..2135 /label=BBa_B0012 promoter 2178..2183 /label=-10 promoter 2158..2163 /label=-35 promoter 2144..2192 /label=BBa_R1075 misc_feature 2205..2208 /label=conserved RBS 2201..2212 /label=BBa_B0034 CDS 2219..2929 /label=cI 857 misc_feature 2930..2962 /label=LVA CDS 2219..2968 /label=BBa_C0081 stem_loop 2988..3031 /label=stem_loop misc_feature 2977..3056 /label=BBa_B0010 terminator 2977..3056 /label=BBa_B0010 stem_loop 3072..3091 /label=T7 TE misc_feature 3098..3098 /label=stop polyA_site 3092..3105 /label=polya misc_feature 3065..3105 /label=BBa_B0012 terminator 3065..3105 /label=BBa_B0012 promoter 3151..3156 /label=-10 promoter 3128..3133 /label=-35 misc_feature 3138..3154 /label=OR1 misc_feature 3114..3130 /label=OR2 misc_feature 3114..3162 /label=BBa_R0051 promoter 3114..3162 /label=BBa_R0051 misc_feature 3175..3178 /label=conserved RBS 3171..3182 /label=BBa_B0034 CDS 3189..3950 /label=YFP (LVA) misc_feature 3945..3950 /label=2 misc_feature 3906..3944 /label=SsrA misc_feature 3430..3430 /label=A (Q->L) misc_feature 3189..3950 /label=BBa_E0032 misc_feature 3189..3950 /label=BBa_E0032 stem_loop 3970..4013 /label=stem_loop misc_feature 3959..4038 /label=BBa_B0010 terminator 3959..4038 /label=BBa_B0010 stem_loop 4054..4073 /label=T7 TE misc_feature 4080..4080 /label=stop polyA_site 4074..4087 /label=polya misc_feature 4047..4087 /label=BBa_B0012 terminator 4047..4087 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac 1981 gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2041 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2101 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttaaatt tcctcttttc 2161 aggccggaat aactccctat aatgcgccac catactagag aaagaggaga aatactagat 2221 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc 2281 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat 2341 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta 2401 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat 2461 cgccacagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga 2521 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcaccta agcttagaac 2581 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc 2641 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt 2701 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg 2761 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca 2821 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc 2881 cgttgtgggg aaagttatgg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga 2941 cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg 3001 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 3061 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtaacacc 3121 gtgcgtgttg actattttac ctctggcggt gataatggtt gctactagag aaagaggaga 3181 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 3241 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 3301 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 3361 ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc 3421 acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 3481 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 3541 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 3601 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 3661 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 3721 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 3781 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 3841 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 3901 acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc 3961 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4021 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 4081 gtttata //