LOCUS       BBa_I2024               2040 bp    DNA     linear       06-May-2024
DEFINITION  LasI Sender Test (I0500.B0034.C0078.B0015)
ACCESSION   BBa_I2024
VERSION     BBa_I2024.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1839
                     /label=LasI
     misc_feature    1840..1872
                     /label=LVA
     misc_feature    1879..1903
                     /label=Help:Barcodes
     CDS             1237..1878
                     /label=BBa_C0078
     stem_loop       1923..1966
                     /label=stem_loop
     misc_feature    1912..1991
                     /label=BBa_B0010
     terminator      1912..1991
                     /label=BBa_B0010
     stem_loop       2007..2026
                     /label=T7 TE
     misc_feature    2033..2033
                     /label=stop
     polyA_site      2027..2040
                     /label=polya
     misc_feature    2000..2040
                     /label=BBa_B0012
     terminator      2000..2040
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga tcgttcagat cggtcgtcgt
     1261 gaagagttcg acaaaaaact gctgggtgaa atgcacaaac tgcgtgctca ggttttcaaa
     1321 gaacgtaaag gttgggacgt ttccgttatc gacgaaatgg aaatcgacgg ttacgacgct
     1381 ctgtccccgt actacatgct gatccaggaa gacaccccgg aagctcaggt tttcggttgc
     1441 tggcgtatct tcgacaccac cggtccgtac atgctgaaaa acaccttccc ggaactgctg
     1501 cacggtaaag aagctccgtg ctccccgcac atctgggaac tgtcccgttt cgctatcaac
     1561 tccggtcaga aaggttccct gggtttctcc gactgcaccc tggaagctat gcgtgctctg
     1621 gctcgttact ccttgcagaa cgacatccag accctggtta ccgttaccac cgttggtgtt
     1681 gaaaaaatga tgatccgtgc tggtctggac gtttcccgtt tcggtccgca cctgaaaatc
     1741 ggtatcgaac gtgctgttgc tctgcgtatc gaactgaacg ctaaaaccca gatcgctctg
     1801 tacggtggtg ttctggttga acagcgtctg gctgtttccg ctgctaacga cgaaaactac
     1861 gctctggttg cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc
     1921 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1981 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//