LOCUS BBa_I20241 3868 bp DNA linear 22-Dec-2024 DEFINITION Double protein generator of lacI and T7 RNAP ACCESSION BBa_I20241 VERSION BBa_I20241.1 FEATURES Location/Qualifiers RBS 1..13 /label=RBS-3\Weak RBS 7..10 /label=RBS misc_feature 1..13 /label=BBa_B0032 RBS 1..13 /label=BBa_B0032 misc_feature 1106..1130 /label=Help:Barcodes CDS 20..1130 /label=BBa_C0013 misc_feature 1..1130 /label=BBa_S03690 RBS 1139..1151 /label=RBS-3\Weak RBS 1145..1148 /label=RBS misc_feature 1139..1151 /label=BBa_B0032 RBS 1139..1151 /label=BBa_B0032 misc_feature 1..1151 /label=BBa_S03746 promoter 3655..3682 /label=putative E. coli promoter CDS 1158..3812 /label=BBa_I2032 terminator 3821..3868 /label=BBa_B0016 misc_feature 1158..3868 /label=BBa_S03693 ORIGIN 1 tcacacagga aagtactaga tgaaaccagt aacgttatac gatgtcgcag agtatgccgg 61 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac 121 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca 181 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca 241 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt 301 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct 361 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat 421 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac 481 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct 541 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc 601 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc 661 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa 721 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat 781 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga 841 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg 901 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa 961 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac 1021 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 1081 ccgactggaa agcgggcagt aataacactg atagtgctag tgtagatcac tactagagtc 1141 acacaggaaa gtactagatg aacacgatta acatcgctaa gaacgacttc tctgacatcg 1201 aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg 1261 aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt 1321 ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca 1381 ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta 1441 agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg gaagccgtag 1501 cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc 1561 aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc 1621 gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag 1681 ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc 1741 tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac 1801 gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg 1861 gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg 1921 caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc 1981 ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg 2041 cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg 2101 aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc 2161 tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga 2221 ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca 2281 ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc 2341 gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct 2401 ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc 2461 aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg 2521 aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt 2581 tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt 2641 ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct 2701 gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg 2761 cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag 2821 gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg 2881 ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag 2941 ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg 3001 cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca 3061 tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca 3121 ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg 3181 gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag 3241 caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga 3301 ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg 3361 tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt 3421 tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg 3481 agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg 3541 tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg 3601 gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca 3661 catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg 3721 agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca 3781 tcttagagtc ggacttcgcg ttcgcgtaat aatactagag ctagcataac cccttggggc 3841 ctctaaacgg gtcttgaggg gttttttg //