LOCUS BBa_I20242 3796 bp DNA linear 25-Nov-2024 DEFINITION AHL Induced expression of T7 RNAP ACCESSION BBa_I20242 VERSION BBa_I20242.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 RBS 1070..1082 /label=RBS-3\Weak RBS 1076..1079 /label=RBS misc_feature 1070..1082 /label=BBa_B0032 RBS 1070..1082 /label=BBa_B0032 promoter 3586..3613 /label=putative E. coli promoter CDS 1089..3740 /label=BBa_I2032 terminator 3749..3796 /label=BBa_B0016 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cacacaggaa 1081 agtactagat gaacacgatt aacatcgcta agaacgactt ctctgacatc gaactggctg 1141 ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg 1201 cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc 1261 aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac 1321 tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca 1381 agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca 1441 ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag 1501 caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg 1561 aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct 1621 acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg 1681 gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg 1741 agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag 1801 gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg 1861 caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt 1921 ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc 1981 gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca 2041 aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg 2101 ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg 2161 aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga 2221 aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc 2281 ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt 2341 acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg 2401 atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact 2461 actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc 2521 gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg 2581 agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt 2641 acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg 2701 ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg 2761 cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag 2821 tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga 2881 ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg 2941 gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg 3001 cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag 3061 ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg 3121 ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact 3181 ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga 3241 ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg 3301 aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac 3361 agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta 3421 tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg 3481 cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc 3541 cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt 3601 cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat 3661 tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt 3721 cggacttcgc gttcgcgtaa tactagagct agcataaccc cttggggcct ctaaacgggt 3781 cttgaggggt tttttg //