BBa_I20243
type: ComponentDefinition
persistent identity: BBa I20243
display id: BBa_I20243
version: 1
was generated by: iGEM to SBOL conversion
description: Autoregulated synthesis of T7 RNAP with lacI negative feedback
created: 2007-07-25T11:00:00Z
modified: 2015-08-31T04:07:39Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: One portion of a virtual machine. Autoregulated synthesis of T7 RNAP. A lacI negative feedback loop is included to maintain a stable level of T7 RNAP
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: na/
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I20241
component: BBa_R0184
sequence annotation: BBa_R0184
sequence annotation: BBa_I20241
sequence: https://synbiohub.org/public/igem/BBa_I20243_sequence/1

BBa_I20241
type: Component
persistent identity: component2260912
display id: component2260912
version: 1
top level: BBa_I20243
definition: BBa_I20241
access: public

BBa_R0184
type: Component
persistent identity: component2260897
display id: component2260897
version: 1
top level: BBa_I20243
definition: BBa_R0184
access: public

BBa_R0184
type: SequenceAnnotation
persistent identity: annotation2260897
display id: annotation2260897
version: 1
top level: BBa_I20243
location: https://synbiohub.org/public/igem/BBa_I20243/annotation2260897/range2260897/1
component: BBa_R0184

https://synbiohub.org/public/igem/BBa_I20243/annotation2260897/range2260897/1
type: Range
persistent identity: range2260897
display id: range2260897
version: 1
top level: BBa_I20243
direction: direction/reverse
start: 1
end: 44
orientation: inline

BBa_I20241
type: SequenceAnnotation
persistent identity: annotation2260912
display id: annotation2260912
version: 1
top level: BBa_I20243
location: https://synbiohub.org/public/igem/BBa_I20243/annotation2260912/range2260912/1
component: BBa_I20241

https://synbiohub.org/public/igem/BBa_I20243/annotation2260912/range2260912/1
type: Range
persistent identity: range2260912
display id: range2260912
version: 1
top level: BBa_I20243
direction: direction/reverse
start: 53
end: 3920
orientation: inline

BBa_I20241
type: ComponentDefinition
persistent identity: BBa I20241
display id: BBa_I20241
version: 1
was generated by: iGEM to SBOL conversion
description: Double protein generator of lacI and T7 RNAP
created: 2007-07-19T11:00:00Z
modified: 2015-08-31T04:07:39Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: Requires a PoPS input to produce lacI and T7 RNAP on a polycistronic transcript. The same strong RBS regulates synthesis of both proteins. The downstream terminator is specific to the T7 RNAP
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_S03693
component: BBa_S03746
sequence annotation: BBa_S03746
sequence annotation: BBa_S03693
sequence: https://synbiohub.org/public/igem/BBa_I20241_sequence/1

BBa_S03693
type: Component
persistent identity: component2252813
display id: component2252813
version: 1
top level: BBa_I20241
definition: BBa_S03693
access: public

BBa_S03746
type: Component
persistent identity: component2252809
display id: component2252809
version: 1
top level: BBa_I20241
definition: BBa_S03746
access: public

BBa_S03746
type: SequenceAnnotation
persistent identity: annotation2252809
display id: annotation2252809
version: 1
top level: BBa_I20241
location: https://synbiohub.org/public/igem/BBa_I20241/annotation2252809/range2252809/1
component: BBa_S03746

https://synbiohub.org/public/igem/BBa_I20241/annotation2252809/range2252809/1
type: Range
persistent identity: range2252809
display id: range2252809
version: 1
top level: BBa_I20241
direction: direction/reverse
start: 1
end: 1151
orientation: inline

BBa_S03693
type: SequenceAnnotation
persistent identity: annotation2252813
display id: annotation2252813
version: 1
top level: BBa_I20241
location: https://synbiohub.org/public/igem/BBa_I20241/annotation2252813/range2252813/1
component: BBa_S03693

https://synbiohub.org/public/igem/BBa_I20241/annotation2252813/range2252813/1
type: Range
persistent identity: range2252813
display id: range2252813
version: 1
top level: BBa_I20241
direction: direction/reverse
start: 1158
end: 3868
orientation: inline

BBa_R0184
type: ComponentDefinition
persistent identity: BBa R0184
display id: BBa_R0184
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (lacI repressible)
created: 2007-04-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
owned by: james
owned by: myers
mutable description: A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0183 fragment
sequence annotation: lacI binding site
sequence annotation: Center of binding site symmetry
sequence: https://synbiohub.org/public/igem/BBa_R0184_sequence/1

BBa_R0183 fragment
type: SequenceAnnotation
persistent identity: annotation1932678
display id: annotation1932678
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
type: Range
persistent identity: range1932678
display id: range1932678
version: 1
top level: BBa_R0184
direction: direction/forward
start: 1
end: 20
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1932679
display id: annotation1932679
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
type: Range
persistent identity: range1932679
display id: range1932679
version: 1
top level: BBa_R0184
direction: direction/forward
start: 20
end: 44
orientation: inline

Center of binding site symmetry
type: SequenceAnnotation
persistent identity: annotation1932680
display id: annotation1932680
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
type: Range
persistent identity: range1932680
display id: range1932680
version: 1
top level: BBa_R0184
direction: direction/forward
start: 31
end: 31
orientation: inline

BBa_S03693
type: ComponentDefinition
persistent identity: BBa S03693
display id: BBa_S03693
version: 1
was generated by: iGEM to SBOL conversion
description: I2032:B0016
created: 2007-05-13T11:00:00Z
modified: 2015-05-08T01:14:29Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _84_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I2032
component: BBa_B0016
sequence annotation: BBa_I2032
sequence annotation: BBa_B0016
sequence: https://synbiohub.org/public/igem/BBa_S03693_sequence/1

BBa_I2032
type: Component
persistent identity: component2218388
display id: component2218388
version: 1
top level: BBa_S03693
definition: BBa_I2032
access: public

BBa_B0016
type: Component
persistent identity: component2218389
display id: component2218389
version: 1
top level: BBa_S03693
definition: BBa_B0016
access: public

BBa_I2032
type: SequenceAnnotation
persistent identity: annotation2218388
display id: annotation2218388
version: 1
top level: BBa_S03693
location: https://synbiohub.org/public/igem/BBa_S03693/annotation2218388/range2218388/1
component: BBa_I2032

https://synbiohub.org/public/igem/BBa_S03693/annotation2218388/range2218388/1
type: Range
persistent identity: range2218388
display id: range2218388
version: 1
top level: BBa_S03693
direction: direction/reverse
start: 1
end: 2655
orientation: inline

BBa_B0016
type: SequenceAnnotation
persistent identity: annotation2218389
display id: annotation2218389
version: 1
top level: BBa_S03693
location: https://synbiohub.org/public/igem/BBa_S03693/annotation2218389/range2218389/1
component: BBa_B0016

https://synbiohub.org/public/igem/BBa_S03693/annotation2218389/range2218389/1
type: Range
persistent identity: range2218389
display id: range2218389
version: 1
top level: BBa_S03693
direction: direction/reverse
start: 2664
end: 2711
orientation: inline

BBa_S03746
type: ComponentDefinition
persistent identity: BBa S03746
display id: BBa_S03746
version: 1
was generated by: iGEM to SBOL conversion
description: S03690:B0032
created: 2007-07-16T11:00:00Z
modified: 2015-05-08T01:14:29Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _84_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0032
component: BBa_S03690
sequence annotation: BBa_S03690
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_S03746_sequence/1

BBa_B0032
type: Component
persistent identity: component2245582
display id: component2245582
version: 1
top level: BBa_S03746
definition: BBa_B0032
access: public

BBa_S03690
type: Component
persistent identity: component2245580
display id: component2245580
version: 1
top level: BBa_S03746
definition: BBa_S03690
access: public

BBa_S03690
type: SequenceAnnotation
persistent identity: annotation2245580
display id: annotation2245580
version: 1
top level: BBa_S03746
location: https://synbiohub.org/public/igem/BBa_S03746/annotation2245580/range2245580/1
component: BBa_S03690

https://synbiohub.org/public/igem/BBa_S03746/annotation2245580/range2245580/1
type: Range
persistent identity: range2245580
display id: range2245580
version: 1
top level: BBa_S03746
direction: direction/reverse
start: 1
end: 1130
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2245582
display id: annotation2245582
version: 1
top level: BBa_S03746
location: https://synbiohub.org/public/igem/BBa_S03746/annotation2245582/range2245582/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_S03746/annotation2245582/range2245582/1
type: Range
persistent identity: range2245582
display id: range2245582
version: 1
top level: BBa_S03746
direction: direction/forward
start: 1139
end: 1151
orientation: inline

BBa_S03690
type: ComponentDefinition
persistent identity: BBa S03690
display id: BBa_S03690
version: 1
was generated by: iGEM to SBOL conversion
description: B0032:C0013
created: 2007-05-12T11:00:00Z
modified: 2015-05-08T01:14:28Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _84_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0032
component: BBa_C0013
sequence annotation: BBa_B0032
sequence annotation: BBa_C0013
sequence: https://synbiohub.org/public/igem/BBa_S03690_sequence/1

BBa_B0032
type: Component
persistent identity: component2243889
display id: component2243889
version: 1
top level: BBa_S03690
definition: BBa_B0032
access: public

BBa_C0013
type: Component
persistent identity: component2243892
display id: component2243892
version: 1
top level: BBa_S03690
definition: lacI
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2243889
display id: annotation2243889
version: 1
top level: BBa_S03690
location: https://synbiohub.org/public/igem/BBa_S03690/annotation2243889/range2243889/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_S03690/annotation2243889/range2243889/1
type: Range
persistent identity: range2243889
display id: range2243889
version: 1
top level: BBa_S03690
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0013
type: SequenceAnnotation
persistent identity: annotation2243892
display id: annotation2243892
version: 1
top level: BBa_S03690
location: https://synbiohub.org/public/igem/BBa_S03690/annotation2243892/range2243892/1
component: BBa_C0013

https://synbiohub.org/public/igem/BBa_S03690/annotation2243892/range2243892/1
type: Range
persistent identity: range2243892
display id: range2243892
version: 1
top level: BBa_S03690
direction: direction/reverse
start: 20
end: 1130
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_I2032
type: ComponentDefinition
persistent identity: BBa I2032
display id: BBa_I2032
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA polymerase
created: 2007-04-11T11:00:00Z
modified: 2016-10-14T06:52:19Z
mutable provenance: Bacteriophage T7
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP). This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to E. coli RNAP.
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 27399
owner id: 135
owning group id: 84
sample status: In stock
status: status/Available
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: putative E. coli promoter
sequence: https://synbiohub.org/public/igem/BBa_I2032_sequence/1

putative E. coli promoter
type: SequenceAnnotation
persistent identity: annotation1928674
display id: annotation1928674
version: 1
top level: BBa_I2032
location: https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
type: Range
persistent identity: range1928674
display id: range1928674
version: 1
top level: BBa_I2032
direction: direction/forward
start: 2498
end: 2525
orientation: inline

BBa_B0016
type: ComponentDefinition
persistent identity: BBa B0016
display id: BBa_B0016
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (T7 RNAP specific, T_Phi)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 gene 1 specific transcriptional terminator
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0016_sequence/1

lacI
type: ComponentDefinition
persistent identity: BBa C0013
display id: BBa_C0013
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor, lacI
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
mutable description: LacI repressor protein, without SsrA degradation tag. No Barcode.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yong-Su Jin (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0013_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214005
display id: annotation2214005
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
type: Range
persistent identity: range2214005
display id: range2214005
version: 1
top level: lacI
direction: direction/forward
start: 1087
end: 1111
orientation: inline

https://synbiohub.org/public/igem/BBa_I20243_sequence/1
type: Sequence
persistent identity: BBa I20243 sequence
display id: BBa_I20243_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactataggggaattgtgagcggataacaattcctactagagtcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I20241_sequence/1
type: Sequence
persistent identity: BBa I20241 sequence
display id: BBa_I20241_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0184_sequence/1
type: Sequence
persistent identity: BBa R0184 sequence
display id: BBa_R0184_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactataggggaattgtgagcggataacaattcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03693_sequence/1
type: Sequence
persistent identity: BBa S03693 sequence
display id: BBa_S03693_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03746_sequence/1
type: Sequence
persistent identity: BBa S03746 sequence
display id: BBa_S03746_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03690_sequence/1
type: Sequence
persistent identity: BBa S03690 sequence
display id: BBa_S03690_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I2032_sequence/1
type: Sequence
persistent identity: BBa I2032 sequence
display id: BBa_I2032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0016_sequence/1
type: Sequence
persistent identity: BBa B0016 sequence
display id: BBa_B0016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0013_sequence/1
type: Sequence
persistent identity: BBa C0013 sequence
display id: BBa_C0013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z