Autoregulated synthesis of T7 RNAP with lacI negative feedback
One portion of a virtual machine. Autoregulated synthesis of T7 RNAP. A lacI negative feedback loop is included to maintain a stable level of T7 RNAP
Double protein generator of lacI and T7 RNAP
Requires a PoPS input to produce lacI and T7 RNAP on a polycistronic transcript. The same strong RBS regulates synthesis of both proteins. The downstream terminator is specific to the T7 RNAP
This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Bacteriophage T7
Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP). This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to E. coli RNAP.
T7 gene 1 specific transcriptional terminator
LacI repressor protein, without SsrA degradation tag. No Barcode.
Conversion of the iGEM parts registry to SBOL2.1