LOCUS       BBa_I20244               903 bp    DNA     linear       29-Jun-2024
DEFINITION  A device used to characterize promoters throught measurement of 
            GFP expression
ACCESSION   BBa_I20244
VERSION     BBa_I20244.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             7..10
                     /label=RBS
     CDS             20..739
                     /label=GFP protein
     misc_feature    20..739
                     /label=BBa_E0040
     misc_feature    748..827
                     /label=BBa_B0010
     stem_loop       759..802
                     /label=stem_loop
     misc_feature    836..876
                     /label=BBa_B0012
     stem_loop       843..862
                     /label=T7 TE
     polyA_site      863..876
                     /label=polya
     misc_feature    869..869
                     /label=stop
     misc_feature    1..21
                     /label=Eco-Spe BB site
ORIGIN
        1 gaattcgcgg ccgcactagt gtcacacagg aaagtactag atgcgtaaag gagaagaact
       61 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      121 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      181 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      241 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      301 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      361 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      421 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      481 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      541 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      601 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      661 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
      721 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa
      781 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      841 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttataatg
      901 cag
//