BBa_I20247
1
BBa_I20247
A device used to characterize promoters throught measurement of RFP expression
2007-08-14T11:00:00Z
2015-08-31T04:07:39Z
.
Device is based on I2057 and contains a partial biobrick site (EcoR1, Not1, Xba1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter EcoR1 and Spe1, and the result will form a mixed site. This design best simulates spacing in actual construction however, parts cannot be removed after testing.
false
false
_11_
0
2010
11
Not in stock
false
.
false
Jason Kelly, Karen Wong, Nishant Bhat
annotation1941353
1
stop
range1941353
1
855
855
annotation1941350
1
BBa_B0012
range1941350
1
822
862
annotation1941344
1
RBS
range1941344
1
7
10
annotation1941351
1
T7 TE
range1941351
1
829
848
annotation1941343
1
BBa_B0032
range1941343
1
1
13
annotation1941346
1
BBa_E1010
range1941346
1
20
700
annotation1941347
1
barcode
range1941347
1
701
725
annotation1941349
1
stem_loop
range1941349
1
745
788
annotation1941348
1
BBa_B0010
range1941348
1
734
813
annotation1941345
1
mrfp1
range1941345
1
20
694
annotation1941352
1
polya
range1941352
1
849
862
annotation1941342
1
EcoR1-Not1-Xba1
range1941342
1
1
21
BBa_I20247_sequence
1
gaattcgcggccgctctagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataatgcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z