LOCUS       BBa_I20248               942 bp    DNA     linear       22-Nov-2024
DEFINITION  Device used to characterize Ribosome Binding Sites through 
            measurement of GFP expression
ACCESSION   BBa_I20248
VERSION     BBa_I20248.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     CDS             61..780
                     /label=GFP protein
     misc_feature    61..780
                     /label=BBa_E0040
     misc_feature    789..868
                     /label=BBa_B0010
     stem_loop       800..843
                     /label=stem_loop
     misc_feature    877..917
                     /label=BBa_B0012
     stem_loop       884..903
                     /label=T7 TE
     polyA_site      904..917
                     /label=polya
     misc_feature    910..910
                     /label=stop
ORIGIN
        1 gaattgtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 gaattcgcgg ccgctctaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      121 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      181 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      241 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      301 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      361 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      421 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      481 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      541 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      601 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      661 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      721 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      781 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttataatgc ag
//