BBa_I20248 1 BBa_I20248 Device used to characterize Ribosome Binding Sites through measurement of GFP expression 2007-08-14T11:00:00Z 2015-08-31T04:07:39Z . Device is based on I2055 and contains a partial biobrick site (EcoR1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1 and the kit EcoR1 and Xba1. The result will form a full BB prefix upstream of the RBS and a mixed site downstream. The full BB prefix has a larger spacing than would occur in actual construction. false false _11_ 0 2010 11 Not in stock false . false Jason Kelly, Karen Wong, Nishant Bhat annotation1941265 1 T7 TE range1941265 1 884 903 annotation1941256 1 BBa_R0040 range1941256 1 1 54 annotation1941264 1 BBa_B0012 range1941264 1 877 917 annotation1941257 1 -35 range1941257 1 20 25 annotation1941259 1 -10 range1941259 1 43 48 annotation1941267 1 stop range1941267 1 910 910 annotation1941258 1 TetR 2 range1941258 1 26 44 annotation1941263 1 stem_loop range1941263 1 800 843 annotation1941260 1 GFP protein range1941260 1 61 780 annotation1941262 1 BBa_B0010 range1941262 1 789 868 annotation1941266 1 polya range1941266 1 904 917 annotation1941261 1 BBa_E0040 range1941261 1 61 780 annotation1941255 1 TetR 1 range1941255 1 1 19 BBa_I20248_sequence 1 gaattgtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgaattcgcggccgctctagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataatgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z