LOCUS       BBa_I20249               943 bp    DNA     linear       28-Jun-2024
DEFINITION  Device used to characterize Ribosome Binding Sites through 
            measurement of GFP expression
ACCESSION   BBa_I20249
VERSION     BBa_I20249.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     CDS             61..780
                     /label=GFP protein
     misc_feature    61..780
                     /label=BBa_E0040
     misc_feature    789..868
                     /label=BBa_B0010
     stem_loop       800..843
                     /label=stem_loop
     misc_feature    877..917
                     /label=BBa_B0012
     stem_loop       884..903
                     /label=T7 TE
     polyA_site      904..917
                     /label=polya
     misc_feature    910..910
                     /label=stop
ORIGIN
        1 gaattgtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 tactagtgcg gccgctctag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      901 actggctcac cttcgggtgg gcctttctgc gtttataatg cag
//