BBa_I20249 1 BBa_I20249 Device used to characterize Ribosome Binding Sites through measurement of GFP expression 2007-08-14T11:00:00Z 2015-08-31T04:07:39Z . Device is based on I2055 and contains a partial biobrick site (Spe1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter Xba1 and Spe1 and the kit EcoR1 and Xba1. Use .5ul of each restriction enzyme during the ligation to ensure the formation of two mixed sites. This design provides the proper spacing, identical to actual construction conditions. false false _11_ 0 2010 11 Not in stock false . false Jason Kelly, Karen Wong, Nishant Bhat annotation1941277 1 BBa_B0012 range1941277 1 877 917 annotation1941280 1 stop range1941280 1 910 910 annotation1941271 1 TetR 2 range1941271 1 26 44 annotation1941279 1 polya range1941279 1 904 917 annotation1941278 1 T7 TE range1941278 1 884 903 annotation1941276 1 stem_loop range1941276 1 800 843 annotation1941272 1 -10 range1941272 1 43 48 annotation1941275 1 BBa_B0010 range1941275 1 789 868 annotation1941273 1 GFP protein range1941273 1 61 780 annotation1941270 1 -35 range1941270 1 20 25 annotation1941268 1 TetR 1 range1941268 1 1 19 annotation1941274 1 BBa_E0040 range1941274 1 61 780 annotation1941269 1 BBa_R0040 range1941269 1 1 54 BBa_I20249_sequence 1 gaattgtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagtgcggccgctctagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataatgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z