LOCUS       BBa_I2025               1926 bp    DNA     linear       22-Dec-2024
DEFINITION  LasR Receiver Test (R1075.B0034.C0079.B0015.R0079.E0022)
ACCESSION   BBa_I2025
VERSION     BBa_I2025.1  
FEATURES             Location/Qualifiers
     promoter        35..40
                     /label=-10
     promoter        15..20
                     /label=-35
     promoter        1..49
                     /label=BBa_R1075
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..792
                     /label=lasR
     misc_feature    793..825
                     /label=LVA
     misc_feature    555..555
                     /label=G
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             76..831
                     /label=BBa_C0079
     stem_loop       876..919
                     /label=stem_loop
     misc_feature    865..944
                     /label=BBa_B0010
     terminator      865..944
                     /label=BBa_B0010
     stem_loop       960..979
                     /label=T7 TE
     misc_feature    986..986
                     /label=stop
     polyA_site      980..993
                     /label=polya
     misc_feature    953..993
                     /label=BBa_B0012
     terminator      953..993
                     /label=BBa_B0012
     misc_feature    1047..1064
                     /label=OP2
     misc_feature    1107..1126
                     /label=OP1
     promoter        1118..1123
                     /label=-35
     promoter        1141..1146
                     /label=-10
     promoter        1002..1158
                     /label=BBa_R0079
     CDS             1165..1926
                     /label=CFP (LVA)
     misc_feature    1883..1920
                     /label=SsrA
     misc_feature    1921..1926
                     /label=2
     misc_feature    1233..1233
                     /label=A
     misc_feature    1165..1926
                     /label=BBa_E0022
     misc_feature    1165..1926
                     /label=BBa_E0022
ORIGIN
        1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa
       61 gaggagaaat actagatggc cttggttgac ggttttcttg agctggaacg ctcaagtgga
      121 aaattggagt ggagcgccat cctccagaag atggcgagcg accttggatt ctcgaagatc
      181 ctgttcggcc tgttgcctaa ggacagccag gactacgaga acgccttcat cgtcggcaac
      241 tacccggccg cctggcgcga gcattacgac cgggctggct acgcgcgggt cgacccgacg
      301 gtcagtcact gtacccagag cgtactgccg attttctggg aaccgtccat ctaccagacg
      361 cgaaagcagc acgagttctt cgaggaagcc tcggccgccg gcctggtgta tgggctgacc
      421 atgccgctgc atggtgctcg cggcgaactc ggcgcgctga gcctcagcgt ggaagcggaa
      481 aaccgggccg aggccaaccg tttcatagag tcggtcctgc cgaccctgtg gatgctcaag
      541 gactacgcac tgcaaagcgg tgccggactg gccttcgaac atccggtcag caaaccggtg
      601 gttctgacca gccgggagaa ggaagtgttg cagtggtgcg ccatcggcaa gaccagttgg
      661 gagatatcgg ttatctgcaa ctgctcggaa gccaatgtga acttccatat gggaaatatt
      721 cggcggaagt tcggtgtgac ctcccgccgc gtagcggcca ttatggccgt taatttgggt
      781 cttattactc tcgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag
      841 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      961 gctcaccttc gggtgggcct ttctgcgttt atatactaga ggcccctcgc tgagcgcgtc
     1021 ccggagctgg gggcaaccta gctgccacct gcttttctgc tagctattcc agcgaaaaca
     1081 tacagatttc cggcgaaatc aaggctacct gccagttctg gcaggtttgg ccgcgggttc
     1141 tttttggtac acgaaagcta ctagatggtg agcaagggcg aggagctgtt caccggggtg
     1201 gtgcccatcc tggtcgagct ggacggcgac gtgaacggcc acaagttcag cgtgtccggc
     1261 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1321 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
     1381 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1441 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1501 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1561 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat
     1621 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     1681 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1741 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1801 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1861 ggcatggacg agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct
     1921 taataa
//