##gff-version 3
##sequence-region BBa_I2025 1 1926
BBa_I2025	.	CDS	76	831	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_I2025	.	tag	793	825	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_I2025	.	engineered_tag	832	856	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_I2025	.	CDS	76	792	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_I2025	.	sequence_alteration	555	555	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_I2025	.	promoter	1	49	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I2025	.	promoter	15	20	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I2025	.	promoter	35	40	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I2025	.	terminator	865	944	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I2025	.	engineered_region	865	944	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2025	.	stem_loop	876	919	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2025	.	engineered_region	1165	1926	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I2025	.	point_mutation	1233	1233	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I2025	.	stop_codon	1921	1926	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I2025	.	CDS	1165	1926	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I2025	.	tag	1883	1920	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I2025	.	engineered_region	1165	1926	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I2025	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I2025	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2025	.	promoter	1002	1158	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_I2025	.	non_covalent_binding_site	1047	1064	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_I2025	.	promoter	1141	1146	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_I2025	.	promoter	1118	1123	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_I2025	.	non_covalent_binding_site	1107	1126	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_I2025	.	terminator	953	993	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I2025	.	stem_loop	960	979	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2025	.	engineered_region	953	993	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2025	.	stop_codon	986	986	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2025	.	polyA_site	980	993	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_I2025
ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatgg
ccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgag
cgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggc
aactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtaccc
agagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggc
cgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaa
gcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgc
aaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgca
gtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatg
ggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactc
tcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagc
tgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggct
acctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagatggtgagcaagggcgaggag
ctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagg
gcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccac
cctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaag
tccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgagg
tgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggg
gcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaac
ttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacg
gccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatca
catggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaac
gacgaaaactacgctttagtagcttaataa